Analyzing the Growth and Caproic Acid Metabolism Mechanism of Rummeliibacillus suwonensis 3B-1 by Tandem Mass Tag-based Quantitative Proteomics
[Objective]This work aims to elucidate the growth and caproic acid metabolism mechanism of Rummeliibacillus suwonensis from protein level,and to further improve its metabolic capacity.It provides a technical basis for the genetic engineering of R.suwonensis.[Method]The differential expression proteins(DEPs)of strain R.suwonensis 3B-1 were extracted by tandem mass tags(TMT)proteomics in aerobic and anaerobic conditions.Its subcellular localization analysis,Gene Ontology(GO)functional analysis,KEGG signaling pathway annotation analysis,and protein-protein interaction were analyzed.[Result]A total of 810 DEPs were identified,including 423 up-regulated proteins and 387 down-regulated proteins.A total of 725 DEPs were subcellular mapped to 6 items,mainly involving cytoplasmic proteins,cytoplasmic membrane proteins and cellwall proteins.GO enrichment of function analysis indicated that,biological processes such as peptide biosynthesis,translation,and peptide metabolism,molecular functions such as structural constituent and structural molecular activity of ribosomes,and cellular components such as ribosomes and ribonucleoprotein complexes have undergone significant changes.A total of 810 DEPs were annotated into 113 KEGG signaling pathways in KEGG database,which mainly were involved cofactor biosynthesis,two-component system,pentose phosphate pathway,glycolysis/gluconeogenesis and oxidative phosphorylation.The phenylalanine-tRNA ligase β subunit and the riboflavin biosynthesis protein RibD had the highest correlation in the protein interaction network.[Conclusion]Under anaerobic conditions,the expression of pyruvate dehydrogenase and pyruvate kinase in the glycolytic pathway was down-regulated.Additionally,proteins related to amino acid metabolism and biotin protein ligase cofactors were also downregulated.This indicates that the bacterium is better suited for growth in an aerobic conditions.Regarding the synthesis of caproic acid,the expression of acyl-CoA thioesterase was significantly up-regulated.In addition,the glycolysis/gluconeogenesis pathway,the tricarboxylic acid cycle and the pentose phosphate pathway provided sufficient precursors and reduction equivalents for caproic acid synthesis,which jointly promoted caproic acid synthesis.
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