Regulatory Genes Mining Related to Transcriptome Sequencing and Phenolic Metabolism Pathway of Canarium album Fruit with Different Fresh Food Quality
[Objective]The category and content of phenolic compounds in fruits are important quality traits closely related to the nutrition and flavor of Chinese olives(Canarium album).This work is to explore the molecular regulation mechanism of phenolic biosynthesis in Chinese olives.[Method]The fruits of Chinese olives(low-phenol/high-phenol)with significant differences in total phenol content were taken as test materials,and the transcriptome analysis was carried out 80-160 d after flowering,and the differential genes of shikimic acid-hydrolyzed tannin/phenylpropane-flavonoid biosynthesis pathway were characterized,and the differentially expressed genes were analyzed by WGCNA,from which the transcription factors related to phenolic metabolism pathway were mined.[Result]The 296 314 Unigenes were obtained,of which 73%were annotated to the database.A total of 1 628 differentially expressed genes(DEGs)were identified in the mature fruits of four varieties(lines)of Chinese olive.KEGG analysis showed that DEGs was significantly enriched in the"flavonoid biosynthesis"pathway of phenolic metabolism pathway.Furthermore,DEGs of shikimic acid-hydrolyzed tannins/phenylpropane-flavonoids biosynthetic pathway in fruit ripening process was characterized.Combining R2 and P values of each module and character in WGCNA analysis,four key modules were screened out.According to the regulation relationship of genes in modules,MCC topological analysis was used to mine key transcription factors in modules with degree value≥1.A total of 137 transcription factors Unigenes were found to be co-expressed with 30 phenolic synthetic structural genes Unigenes.The functional annotations of the transcription factors Unigenes were from 35 gene families,the most of which were zinc finger proteins(C2C2,C3H,C2H2 and PHD),followed by B3,HB,MYB and NAC gene families.[Conclusion]This study initially analyzed the differences in phenolic metabolic pathways in Chinese olive fresh food quality from a transcriptomic perspective,and concurrently excavated the differential genes regulating phenolic metabolism,which may provide an important basis for further investigation of the molecular mechanisms underlying the differences in Chinese olive fresh food quality.
国家科技期刊平台
NSTL
万方数据
