Identification and Expression Analysis of 14-3-3 Gene Family in Dendrobium officinale
[Objective]14-3-3 protein,also known as general regulatory factor(GRF),encodes through multi-gene family,and plays a central role in encouraging plant growth and development and stress-related adversity responses.The identification of GRF gene family in Dendrobium officinale may provide theoretical basis for the study of D.officinale GRF gene function and genetic improvement.[Method]Bioinformatics was used to identify the members of 14-3-3 gene family,and analyze their physicochemical properties,chromosomal localization,phylogenetic tree,gene structure,and cis-acting elements of each DoGRF member.Simultaneously,their expression profiles in different tissues and under the treatment of low-temperature or salt stress were detected using the real-time quantitative PCR.[Result]Results demonstrated that seventeen DoGRF gene family members were presented in D.officinale,which harbored two subfamilies(ε group and non-ε group).They were unevenly located on 7 chromosomes,and harbored seven tandem repeat gene pairs.The gene structure,conserved motifs,and secondary structure of DoGRF members within the same subfamily were similar.The cis-acting elements at the promoter regions of DoGRF family genes contained abundant hormone and stress response elements.DoGRF genes family were expressed in various tissues(flower buds,sepals,labellum(lip),pollinia,gynostemium(column),stems,leaves,roots,green root tips,and white part of roots),and most of them were highly expressed in floral organs,followed by stems and roots.Besides,they were differentially expressed under treatment of low-temperature or salt-stress,suggesting that they might be involved in regulating the stresses of low-temperature and salinity.Especially,DoGRF2 might play a crucial role in the process of stress responses.[Conclusion]17 members of DoGRF family were systematically identified from D.officinale at the genome level.Different genes possessed different responses to abiotic stresses,and exhibited tissue-specific expression profiles.DoGRF2 showed positively response to low-temperature and salt stress..
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