Detection of NK Cell Cytotoxicity:Real-time Dynamic Imaging-Based Analysis
[Objective]Currently cellular immunotherapy is a fast-growing and reliable means of treating cancer.This study aims to develop a new method of detecting the effects of cell therapy products.[Method]By constructing tumor cells expressing green fluorescent protein(GFP),a method for evaluating the cytotoxicity of cell therapy products was constructed based on a real-time live-cell dynamic imaging system.[Result]The real-time live-cell dynamic imaging system was used to monitor K562-GFP cells,and there was a good linear relationship between the total fluorescence intensity and the number of cells.In co-culture with extremely low effector to target(E∶T)ratio,high accuracy was also achieved.The cytotoxicity of natural killer(NK)cells was accurately evaluated by calculating the half-maximum effector concentration(EC50)of NK cells for K562-GFP cells.Flow cytometry(FCM)was used to detect the correlation between apoptosis of K562-GFP cells and the quenching of cell fluorescent protein,which proved the feasibility of the detection method.[Conclusion]This method can be used to evaluate killing ability of immune cells of different types or production processes at a low cost,without requiring the use of dyes or antibodies.
国家科技期刊平台
NSTL
万方数据
维普
