Identification and Expression Pattern Analysis of GmHMGS Gene in Soybean
[Objective]3-hydroxy-3-methylglutaryl-CoA synthase(HMGS)is a key enzyme in the mevalonate pathway and plays an important role in the growth,development and synthesis of terpenoids.Bioinformatics study and expression pattern analysis of the GmHMGS gene can lay the foundation for exploring the function of GmHMGS gene.[Method]The gene was identified and analysed using bioinformatics approaches,and then its expression pattern was examined using real-time fluorescence quantitative PCR(RT-qPCR).[Result]The GmHMGS gene had six family members in total,named GmHMGS1 to GmHMG6.The analysis of physicochemical properties and gene structure showed that GmHMGS3,GmHMG4,GmHMGS5,GmHMGS6 were stable hydrophilic proteins,and all GmHMGS possessed conserved functional domains of HMG-CoA synthase.Promoter sequence analysis revealed that GmHMGS has hormone and adversity stress related action elements.The analysis of tissue expression pattern revealed that GmHMGS was expressed in roots,stems,leaves,flowers,grains,pod skins,and root nodules,with leaves having the highest relative expression of GmHMGS1,GmHMG2,GmHMG3,GmHMG5,and GmHMG6,and roots having the highest relative expression of GmHMGS4.The GmHMGS genes were responsive to PEG6000,NaCl,and H2O2 stresses,as well as MeJA and ABA exogenous hormones,and the relative expression of GmHMGS1 and GmHMGS6 was higher under a wide range of adversity stresses,according to expression analysis under abiotic stress and different exogenous hormone-induced conditions.[Conclusion]GmHMGS gene family may be involved in response to drought,oxidative and salt stresses in soybean.
国家科技期刊平台
NSTL
万方数据
维普
