Effects of Melatonin on the Fruit Softening and Ethylene Synthesis of Post-harvest Oriental Melon
[Objective]Melatonin plays an important role in post-harvest fruit preservation.To explore the efects of melatonin on the fruit softening and ethylene synthesis of post-harvest oriental melon.[Method]The fruit(Cucumis melo cv.'Huaguniang')were soaked at 0,100,200,300,400 μmol/L melatonin solution,by measuring the firmness,weight loss,decay rate,and endogenous ethylene production,the optimal concentration was selected.At the optimal concentration,the ACC content and the activities of ACO and ACS were studied.Furthermore,the transcriptome sequecing and real-time PCR were used to compare the gene expression of ethylene synthesis,signal transduction pathway and fruit softening between melatonin treatment and control.[Result]200 μmol/L treatment delayed the weight loss rate,slow down firmness,and prevent fruit decay and deterioration effectively.Melatonin treatment decreased the ethylene synthesis of fruit and ACC level,while did not delay the arrival of peak period.The transcriptome sequecing results indicated that there were differentially expressed genes in ethylene synthesis,signal transduction pathways and fruit softening between melatonin and control fruits.Melatonin treatment inhibited the expressions of CmACO1-2,CmACS1,CmERFs,CmEXPs and CmEFRs.[Conclusion]200 μmol/L melatonin delays the weight loss rate,slows down firmness,and decreases the ethylene synthesis,by inhibiting the expressions of genes in ethylene synthesis,signal transduction pathways and fruit softening and prolong the storaage life.
国家科技期刊平台
NSTL
万方数据
维普
