Cloning and Expression Analysis of CsERF025L Transcription Factor in Cucumber
[Objective]ERF transcription factor is a subfamily of AP2/ERF gene family,which plays a key role in plant growth and development and resistance to external stress.Cloning CsERF025-like(CsERF025L)and analyzing its expression pattern may lay a foundation for studying the function of CsERF025L.[Method]CsERF025L from cucumber(Cucumis sativus L.)stem tip was cloned by RT-PCR,and bioinformatics analysis of CsERF025L protein physicochemical properties,subcellular localization and interacting proteins was performed.Real-time fluorescence quantitative PCR was used to analyze the expression characteristics of CsERF025L in cucumber.[Result]The encoding sequence of CsERF025L was 510 bp,encoding 169 amino acids,with an AP2/ERF domain,belonging to the ERF subfamily of the AP2/ERF family.CsERF025L protein was an unstable hydrophobic protein located in the nucleus.CsERF025L protein interacts with members of P450 gene family,BEE3 transcription factor,DIR1 protein and MATE family members.CsERF025L promoter contained light response,hormone response,stress response and other elements.The expressions of CsERF025L in the cucumber seeds was the highest,which was significantly higher than that in leaves,stems and roots.The expression patterns of CsERF025L in the cucumber with different phyllotaxis types were compared,and the expressions of CsERF025L in the stem tip of opposite phyllotaxis cop1 were significantly higher than those of cucumber alternate phyllotaxis R1.[Conclusion]The findings of this study suggest that CsERF025L is a nuclear-localized ERF transcription factor,potentially involved in the formation and regulation of cucumber alternate phyllotaxis.
国家科技期刊平台
NSTL
万方数据
维普
