首页|生防细菌HX0037对栝楼炭疽病的防病能力及其机制

生防细菌HX0037对栝楼炭疽病的防病能力及其机制

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  • 国家科技期刊平台
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  • NSTL
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  • 维普
[目的]研究潜在生防细菌HX0037 对栝楼炭疽病的防病能力,并探究其生防机制.[方法]采用平板对峙法测定HX0037 对栝楼炭疽菌(Colletotrichum gloeosporioides)、枣炭疽菌(Collettrichum coccodes)、梨炭疽菌(Colletotrichum fructicola)和苹果炭疽菌(Cryptosporiopsis malicorticis)的拮抗活性;离体接种法评价其对栝楼叶片炭疽病和果实炭疽病的防治效果;结合形态学、生理生化特征和全基因组的序列分析结果,确定生防细菌的分类地位,并预测其次级代谢产物;采用酸沉淀法提取脂肽类化合物,并对其进行高效液相色谱串联质谱分析(LC-MS);最后通过观察生防细菌所产脂肽对栝楼炭疽病菌菌株生长和菌丝形态的影响,以及检测该菌株产酶和铁载体能力,探究可能的生防机制.[结果]HX0037 菌株对 4 种瓜果炭疽病菌均有不同程度的抑菌活性,其中对栝楼炭疽病菌的抑菌效果最好,其能明显改变病菌细胞膜的通透性;与单独接种病菌的栝楼组相比,接种HX0037菌株能显著减小栝楼叶片炭疽病和栝楼果实炭疽病的病斑面积,其防病效果分别为 61.50%和 52.51%;HX0037 菌株被进一步鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),其基因组中包含 12 个次级代谢产物基因簇,它还具备产生纤维素酶、蛋白酶、淀粉酶和铁载体的能力;从HX0037 代谢物中分离获得的脂肽导致栝楼炭疽病菌的菌丝发生扭曲、膨大等畸变现象,经LC-MS鉴定,该脂肽粗提物中含有表面活性素和丰原素.[结论]解淀粉芽孢杆菌HX0037 具有良好的抑制炭疽病菌生长的能力,有望被进一步开发为生物农药用于栝楼炭疽病的绿色防控.
Efficacy and Its Mechanism of Bacterial Strain HX0037 on the Control of Anthracnose Disease of Trichosanthes kirilowii Maxim
[Objective]The biocontrol ability of bacterium HX0037 against anthracnose disease of Trichosanthes kirilowii Maxim and its biocontrol mechanism were studied.[Method]The antagonistic activities of HX0037 against four pathogens of fruit anthracnose disease were determined in petri dishes experiments.The biocontrol efficiencies of HX0037 against Trichosanthes leaf anthracnose and Trichosanthes fruit anthracnose were evaluated on detached leaves and fruits in vitro.In addition,HX0037 was further identified based on the analyses on morphological features,physiological and biochemical characteristics and molecular biological identification results of the whole genome,and the secondary metabolites of biocontrol bacteria HX0037 were also predicted.The lipopeptide compounds produced by strain HX0037 were extracted by acid precipitation and analyzed by high-performance liquid chromatography tandem mass spectrometry(LC-MS).Finally,the possible biocontrol mechanism was explored through two experiments,observing the effects of lipopeptides produced by strain HX0037 on the hyphal growth and morphology of Colletotrichum gloeosporioides,and the abilities of strain HX0037 to produce enzyme and siderophore.[Result]Strain HX0037 showed varied antimicrobial activity against four pathogens of fruit anthracnose disease,especially the best against C.gloeosporioides,a pathogen of Trichosanthes anthracnose disease,and it obviously changed the cell membrane permeability of the pathogenic fungi.Compared with plant tissues inoculated with the pathogen alone,inoculation with strain HX0037 significantly reduced the incidence of leaf and fruit anthracnose diseases of T.kirilowii Maxim,and the biocontrol efficacy was 61.50%and 52.51%,respectively.Strain HX0037 was further identified as Bacillus amyloliquefaciens,and its genome contained 12 secondary metabolite gene clusters,and it decomposed the protein,cellulose and starch,and also produced siderophore.The hyphae of C.gloeosporioides were distorted,intumescent,broken and more transparent after treatment with the antifungal lipopeptide produced by strain HX0037.Surfactin and fengycin were detected from the lipopeptide extracts of strain HX0037 by LC-MS.[Conclusion]The B.amyloliquefaciens HX0037 has strong antagonistic activity on C.gloeosporioides,and is expected to be further developed as a biological agent for the biocontrol of Trichosanthes anthracnose diseases.

Bacillus amyloliquefaciensbiological controlTrichosanthes anthracnose diseaseantagonismwhole genomespecies identificationlipopeptide

徐伟芳、李贺宇、张慧、何仔昂、高文恒、谢紫洋、王传文、尹登科

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安徽中医药大学药学院 中药研究与开发安徽省重点实验室,合肥 230012

安徽中医药大学 安徽中药材种植联合研究中心,合肥 230012

潜山市传文瓜子有限公司,安庆 246300

解淀粉芽孢杆菌 生物防治 栝楼炭疽病 拮抗 全基因组 菌种鉴定 脂肽

安徽省自然科学基金中药研究与开发安徽省重点实验室开放基金大健康研究院安徽中药材种植联合研究中心专项安徽中医药大学-潜山市医药健康发展技术研究中心科研攻关研发项目安徽中医药大学人才支持项目大学生创新创业训练计划省级项目

2108085QH374AKLPDCM202310yjzx20230052021QSHZ012020rcyb008S202210369005

2024

10.13560/j.cnki.biotech.bull.1985.2023-1172

生物技术通报
中国农业科学院农业信息研究所

生物技术通报

CSTPCD北大核心
影响因子：0.505
ISSN：1002-5464
年,卷(期)：2024.40(4)
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