Identification and Functional Study of T6SS Effector Protein PA0423 of Pseudomonas aeruginosa
[Objective]The objective of this study is to investigate the novel effector proteins and their functions in the type VI secretion system(T6SS).[Method]The secretion mode and function of PA0423 were explored by gene knockout,immunoblotting,co-immunoprecipitation,intraspecific and interspecific competition,Escherichia coli toxicity assays and crystal violet biofilm assays.[Result]Western blotting and co-immunoprecipitation experiments showed that PA0423 interacted with PA0262,and the secretion of PA0423 depended on H2-T6SS.PA0423 is a H2-T6SS-dependent antimicrobial effector protein with interspecific competitive advantages.PA0423 is toxic to Escherichia coli and PA0422 is its immune protein.PA0423 affected the formation of bacterial biofilms.[Conclusion]PA0423 is a H2-T6SS-dependent antimicrobial effector protein,which has bactericidal function and affects the formation of bacterial biofilms.
Pseudomonas aeruginosatype VI secretion systemPA0423effector proteins
国家科技期刊平台
NSTL
万方数据
维普
