Research Progress in DNA Methylation Sequencing Technology
DNA methylation is an important epigenetic modification that plays a key role in the growth and development of animals and plants,as well as in the occurrence of diseases and the regulation of gene expression.Clinically,DNA methylation tumor markers can serve as biological markers for the diagnosis,prognosis,and treatment of tumors.Accurate detection of DNA methylation is of great significance for elucidating the life mechanisms of biological growth,development,and disease occurrence.DNA methylation sequencing technology is a powerful tool for studying DNA methylation and is widely used for mapping DNA methylation in the genome.In recent years,in order to better detect DNA methylation site information,scientists have proposed a series of high-throughput DNA methylation sequencing technologies,which have improved sequencing detection sensitivity,reduced sequencing costs and experimental expenses,and greatly promoted the development of epigenomics.This article reviews a series of DNA methylation sequencing technologies,focusing on the technical principles and application scenarios of three sequencing technologies:WGBS,TAPS,and EM-seq.It also introduces sequencing methods for locating DNA methylation at the single-cell level,and finally prospects the future development direction.
DNA methylationWGBSTAPSEM-seqsingle cellmultiomics
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