Response of Arabidopsis AtiPGAM2 Gene to Abiotic Stress
[Objective]The AtiPGAM2 gene is a member of the alkaline phosphatase superfamily in Arabidopsis and is involved in the glycolysis process.Studying the response mechanism of AtiPGAM2 gene under stress conditions lays the foundation for further studying the stress resistance function of AtiPGAM2.[Method]This study used PlantCARE online software to analyze the cis-acting elements of the AtiPGAM2 gene promoter.Real time fluorescence quantitative PCR was used to detect the response patterns of AtiPGAM2 under NaCl and ABA stress.The AtiPGAM2 gene was cloned and transferred it into Arabidopsis to obtain an overexpressing strain of the AtiPGAM2 gene.The Atipgam2 mutant was identified using the three primer method.Statistical analysis was conducted on the germination rate,green seedling rate,and phenotypes under various abiotic stresses,including mannitol,ABA,and MeJA,for overexpression,mutants,and wild-type under salt stress.[Result]There are multiple abotic stress and hormone responsive elements on its promoter,such as light,MeJA,low temperature,ABA,SA,GAs,etc.Fluorescence quantitative PCR analysis showed that AtiPGAM2 was induced to varying degrees under NaCl and ABA stress.AtiPGAM2 gene overexpression and mutant strains were obtained successfully.Under salt stress,the germination rate and green seedling rate of AtiPGAM2 overexpressing strains were higher than those of the wild type,while the mutant surface type was the opposite.The germination rate of the mutant under mannitol treatment was lower than that of the wild-type.The germination rates of mutant and overexpressing AtiPGAM2 strains treated with ABA within 48 h were lower than those of the wild-type.Under MeJA treatment,the number of overexpressed lateral roots was higher than that of the wild-type,while the mutant was the opposite.[Conclusion]AtiPGAM2 has the tolerance to salt,and this gene responds to mannitol,ABA,and MeJA treatments.
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