Study on the Microbial Community of Rhizosphere Soil in Ancient Tea Garden and Modern Organic Tea Garden in Jingmai Mountain
[Objective]It is to study the composition and diversity of rhizosphere soil microbial communities in tea gardens of different habitats,to identify the main driving factors affecting rhizosphere soil microbial communities,and to provide theoretical basis for the study of the relationship between rhizosphere soil microbial communities and pest control of tea trees,as well as for the function and utilization of soil microbials.[Method]We used Illumina-MiSeq high-throughput sequencing technology to investigate the community composition and diversity of soil microorganisms in the rhizosphere soil communities of Jingmai Mountain Ancient Tea Garden(GS)and Jingmai Mountain Modern Organic Tea Garden(TS),and used redundancy analysis to explore the relationship between soil nutrients and microorganisms.[Result]The cation exchange capacity,organic matter,humus,ammonia nitrogen,ammonium nitrogen,available phosphorus,and water content in the rhizosphere soil in TS were higher than those in the GS,showing significant differences;the dominant bacterial populations in both gardens were Actinobacteria,Acidobacteria,and Planctomycetes,and the dominant fungal populations are Ascomycota,Mortierellomycota,and Basidiomycota,except for the unclassified genera.The dominant bacterial genera in GS are very similar to those in TS,but the relative abundance of each genus varies greatly in two gardens.Redundancy analysis shows that cation exchange capacity,organic matter,humus,ammonia nitrogen,and ammonium nitrogen were the main environmental factors causing differences in the microbial community structure and diversity in the rhizosphere soil of two tea gardens.[Conclusion]The nutrient and species richness of the rhizosphere soil of TS was significantly higher than that of Gs,and the composition of the dominant bacterial genera in the rhizosphere soil of two tea gardens was highly similar,but their abundance varied significantly,showing a high degree of correlation with soil nutrients.
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