Identification and Induced-expression Analysis of REVEILLE Family in Brassica napus L.
[Objective]REVEILLE family genes have important biological functions,but there are few studies on this family gene in Brassica napus L.This project was conducted to further understand the function of RVE family genes in B.napus and their relationship with abiotic stress.[Method]Bioinformatics methods were used to identify33,16 and 16 RVE genes in B.napus,B.rapa and B.oleracea,and to analyze their phylogenetic development,chromosome localization,gene structure,physical and chemical properties,and promoter cis-acting elements.[Result]All RVE proteins were classified into two subfamilies.The 33 BnaRVE were located on 18 chromosomes of the A sub-genome and 15 chromosomes of the C sub-genome.Most members belonged to relatively stable hydrophobic proteins.Most of the Ka/Ks values were<1,indicating that the family was subjected to strong purification selection.The gene structure varied greatly,and the number of introns ranged from 4 to 11.Collinearity analysis showed that there were large number of homologous genes in B.napus and Arabidopsis,B.rapa and B.oleracea.Most RVE family members of B.napus expressed RVE in the cotyledon and leaves,and the expression was the highest.BnaRVE promoter contained a large number of cis-acting elements related to hormone and biological stress.Through quantitative PCR analysis,the expressions of 4 BnaRVE genes were significantly upregulated under ABA and MeJA induction as well as low temperature stress.[Conclusion]Thirty-three members of the BnaRVE family were identified in the genome of B.napus.Different genes showed different expression patterns in different developmental stages and different tissues.Different genes have different expression patterns in response to various stresses.RVE family members have positive response function to ABA,MeJA and cold stress.
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