Mechanism Study and Application of CRISPR/Cas12a-based Biosensing Platform
CRISPR/Cas12a system can accurately identify specific single-stranded DNA or double-stranded DNA containing PAM sequences with programmable guide RNA.Then,the non-specific single strand DNA can be cleaved rapidly at the same time as the target substrate,which shows great prospects in biosensing.In recent years,the CRISPR/Cas12a system has been widely used for assisted detection of biomarkers,and its biomarker sensing platform has been applied in the construction of biomolecular sensors such as visualized cell pathways and in vivo diagnostics.Based on the principles of CRISPR/Cas12a biosensing platform construction,this paper summarizes the variants of CRISPR/Cas12a system under different application scenarios,then highlights and summarizes the applications of in vitro and in vivo sensing platforms based on this system,and further discusses the characteristics of different sensing platforms.Finally,the paper summarizes and prospects the advantages and limitations of current applications,aiming to provide a certain reference for the research and application in this field.
CRISPR/Cas12abiosensing in vivobiosensing in vitroaccurately identifying
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