首页|枯草芽孢杆菌B579对黄瓜枯萎病的防治及其诱导抗性研究

枯草芽孢杆菌B579对黄瓜枯萎病的防治及其诱导抗性研究

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  • 国家科技期刊平台
  • NETL
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[目的]探究枯草芽孢杆菌B579 对黄瓜枯萎病菌的生防效果及诱导抗性机理.[方法]采用平板对峙试验和温室盆栽试验,检测病原菌生长及形态变化、植物生长量、叶片防御酶活性、丙二醛含量、防御相关基因表达量等指标.[结果]枯草芽孢杆菌B579 对黄瓜枯萎病菌具有较强的抑制作用,抑菌圈周围菌丝出现粗细不均、膨胀、断裂等现象.盆栽试验表明B579 对黄瓜枯萎病的防治效果为 78.80%.B579 处理组黄瓜幼苗的株高、茎粗、鲜重、干重分别比对照组增加了 13.87%、4.17%、15.15%、12.77%.相比单独接种病原菌(FOC)处理,接种 B579 再接种病原菌(B579+FOC)处理在第 4 天和第 7 天显著提高了黄瓜叶片苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,并减少黄瓜叶片丙二醛(MDA)的含量(P<0.05).实时荧光定量PCR 检测结果表明,接种 B579 再接种病原菌后,黄瓜叶片防御相关基因LOX、PR1、PR3 和CAT表达量在第 4 天和第 7 天显著高于其他处理(P<0.05).[结论]枯草芽孢杆菌B579 能够通过直接抑制病原菌生长和使黄瓜产生诱导抗性来有效防治黄瓜枯萎病.
Study on the Control and Induced Resistance in Cucumber with Bacillus subtilis B579 against Cucumber Fusarium Wilt
[Objective]To explore the control effect and mechanism of Bacillus subtilis B579 on cucumber Fusarium wilt.[Method]Plate confrontation test and greenhouse pot experiment were used to detect the growth and morphological changes of the pathogen,plant growth,activity of defense enzymes in leaves and malondialdehyde content,and expression of defense-related related genes.[Result]The B.subtilis B579 had a strong inhibitory effect on cucumber Fusarium wilt,and the mycelium around the inhibition zone showed uneven thickness,swelling and breakage.Pot experiment demonstrated that B579 achieved the disease control efficiency against cucumber wilt,reaching 78.80%.The plant height,stem diameter,fresh weight,and dry weight of cucumber seedlings in B579 treatment increased by 13.87%,4.17%,15.15%and 12.77%compared with the control group,respectively.After pretreatment with B579,pathogen inoculation significantly increased the activities of phenylalanine ammonilyase(PAL),polyphenol oxidase(PPO),peroxidase(POD),and catalase(CAT)and reduced the content of malondialdehyde(MDA)in cucumber leaves on day 4 and 7 compared with the control group.The results of real-time fluorescence quantitative PCR showed that the expressions of LOX,PR1,PR3 and CAT genes in cucumber leaves after inoculation with B579 and then challenge with pathogen were significantly higher than those in other treatments at 4 and 7 d.[Conclusion]B.subtilis B579 can control cucumber Fusarium wilt by directly inhibiting the growth of pathogen and inducing resistance in cucumber.

Bacillus subtilis B579promoting growthcucumber Fusarium wiltinduced resistancebiological control

范宗强、冯靖涵、郑丽雪、王硕、彭向前、陈芳

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聊城大学药学院,聊城 252059

枯草芽孢杆菌B579 促生 黄瓜枯萎病 诱导抗性 生物防治

国家自然科学基金项目山东省自然科学基金项目山东省研究生教育教学改革研究重点项目山东省抗体制药协同创新中心开放课题聊城大学畜牧学重点学科开放课题

31401799ZR2020MC126SDYJG19056CICGAD1816319462207-23

2024

10.13560/j.cnki.biotech.bull.1985.2024-0160

生物技术通报
中国农业科学院农业信息研究所

生物技术通报

CSTPCD北大核心
影响因子：0.505
ISSN：1002-5464
年,卷(期)：2024.40(7)