Analysis of Differential Metabolites and Bacterial Community Structure in the Soils of a Pineapple Orchard under Different Mulching Treatments
[Objective]The aim of this work is to investigate the effects of different mulching treatments on the soil microecological environment in pineapple orchard.[Method]Taking the un-mulched soil as the control(CK),the differences in physicochemical properties,microbial abundance,bacterial community structure,and metabolites of the soils of mulching treatments with polyethylene mulch(PM),non-woven fabric(NW)and biodegradable film(BF)were compared and measured,respectively.[Result]Compared with CK,PM significantly increased the activity of protease,whereas,decreased the activities of catalase and sucrase.NW significantly increased the content of available P,and decreased the activities of catalase and sucrase.Similarly,BF significantly increased the contents of total N,available P,and the activity of acid phosphatase,and decreased soil pH and the activities of catalase and sucrase.Compared with CK,PM significantly increased the community of actinomycetes.NW significantly increased the community of bacteria,while decreased the actinomycetes.BF significantly increased the communities of bacteria and fungi,while decreased the actinomycetes.The bacterial 16S high-throughput sequencing profiling suggested that the diversity of CK was higher than those of the three mulching treatments,and the bacteria abundance of BF was the highest among the three treatments and CK.Metabolites analysis indicated that there were 17 differential accumulated metabolites in the soils of three comparison groups with CK,mainly including carbohydrates,acids,nitrogen compounds and alcohols.And BF accumulated the highest sugar metabolites in the three mulching treatments.CCA analysis showed that soil pH affected the differentiation of bacterial community structure,and soil nutrients and enzyme activities were positively correlated with bacterial community structure.[Conclusion]Among the three mulching treatments,NW would benefit to maintain the relatively stable microecological environment of soil during the vegetative growth period of pineapple.
mulching-practicespineapplephysiochemical properties of soilsoil metabolitesbacterial community structure
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