摘要
[目的]全面了解绵羊肺炎支原体GH3-3 株的基因序列,研究其潜在的致病机制及其复制、转录、翻译过程的调控机制.[方法]采用体外培养,利用细菌基因组DNA提取试剂盒提取绵羊肺炎支原体GH3-3 株基因组DNA,进行全基因组测序.[结果]绵羊肺炎支原体GH3-3 株基因组大小为 1 060 772 bp,GC含量为 29.66%,基因组组分分析后发现,GH3-3 株的基因组含有 730 个编码基因,总长度为 914 379 bp,平均长度为 1 252.57 bp,占基因组全长的 86.2%.串联重复序列共 149 个,总长为20 926 bp,占基因组全长的 1.97%.微卫星DNA序列 102 个,tRNA 30 个,rRNA 3 个.在NR、SwissProt、GOG、KEGG、GO、CARD、CAZy、PHI、TCDB、RMS数据库中,分别有 719、459、473、394、449、33、5、180、113、59 个基因被注释;在VFDB数据库中,共注释到了 76 个毒力因子相关的基因.将基因组序列提交至NCBI网站,获得登录号为:PRJNA1051969.[结论]获得了绵羊肺炎支原体GH3-3 株完整的基因组信息,预测和注释了其基因的功能,明确了GH3-3 株以及与国内外其他绵羊肺炎支原体菌株之间的遗传进化关系.
Abstract
[Objective]This study is aimed to decode the genomic sequence of Mycoplasma ovipneumoniae strain GH3-3,to investigate its pathogenic potential and to elucidate the regulatory mechanisms of its replication,transcription,and translation.[Method]M.ovipneumoniae GH3-3 strain was cultured in vitro.When it reached the late logarithmic stage of growth,the genomic DNA of M.ovipneumoniae GH3-3 strain was extracted with bacterial genomic DNA extraction kit and sent for whole genome sequencing and commentary.[Result]The genome size of GH3-3 strains of M.ovipneumoniae was 1 060 772 bp,and the GC content was 29.66%.The genome component analysis showed that the genome of GH3-3 strain contained 730 coding genes,with a total length of 914 379 bp and an average length of 1 252.57 bp,accounting for 86.2%of the total genome length.There were 149 tandem repeats with a total length of 20 926 bp,accounting for 1.97%of the total length of the genome.There were 102 microsatellite DNA sequences,30 tRNA sequences,and 3 rRNA sequences.The 719,459,473,394,449,33,5,180,113 and 59 genes were annotated in NR,SwissProt,GOG,KEGG,GO,CARD,CAZy,PHI,TCDB,and RMS databases,respectively.A total of 76 virulence factor-related genes were annotated.The genome sequence was submitted to the NCBI website with the login number:PRJNA1051969.[Conclusion]The complete genome information of M.ovipneumoniae GH3-3 strain was acquired,and the gene function was predicted and annotated.Furthermore,the genetic and evolutionary relationships between GH3-3 strain and other strains of M.ovipneumoniae were elucidated globally.
基金项目
国家重点研发计划(2022YFD1800704)
中国农业科学院兰州兽医研究所"元亨人才"计划项目(所级青年英才培育项目)(NKLS2020-119)
甘肃省科技重大专项课题(23ZDNA007)
国家科技期刊平台
NSTL
万方数据