Whole Genome Sequencing and Bioinformatics Analysis of Mycoplasma ovipneumoniae GH3-3 Strain
[Objective]This study is aimed to decode the genomic sequence of Mycoplasma ovipneumoniae strain GH3-3,to investigate its pathogenic potential and to elucidate the regulatory mechanisms of its replication,transcription,and translation.[Method]M.ovipneumoniae GH3-3 strain was cultured in vitro.When it reached the late logarithmic stage of growth,the genomic DNA of M.ovipneumoniae GH3-3 strain was extracted with bacterial genomic DNA extraction kit and sent for whole genome sequencing and commentary.[Result]The genome size of GH3-3 strains of M.ovipneumoniae was 1 060 772 bp,and the GC content was 29.66%.The genome component analysis showed that the genome of GH3-3 strain contained 730 coding genes,with a total length of 914 379 bp and an average length of 1 252.57 bp,accounting for 86.2%of the total genome length.There were 149 tandem repeats with a total length of 20 926 bp,accounting for 1.97%of the total length of the genome.There were 102 microsatellite DNA sequences,30 tRNA sequences,and 3 rRNA sequences.The 719,459,473,394,449,33,5,180,113 and 59 genes were annotated in NR,SwissProt,GOG,KEGG,GO,CARD,CAZy,PHI,TCDB,and RMS databases,respectively.A total of 76 virulence factor-related genes were annotated.The genome sequence was submitted to the NCBI website with the login number:PRJNA1051969.[Conclusion]The complete genome information of M.ovipneumoniae GH3-3 strain was acquired,and the gene function was predicted and annotated.Furthermore,the genetic and evolutionary relationships between GH3-3 strain and other strains of M.ovipneumoniae were elucidated globally.
国家科技期刊平台
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万方数据
