[Objective]Glutamine synthetase(GS)is a key enzyme in the GS-GOGAT cycle of nitrogen metabolism.This work is aimed to investigate the family members of GS in soybean and the response to external stress.[Method]Based on bioinformatics methods,we comprehensively identified GS genes in soybean(Glycine max),clarified the location and structure of soybean GmGSs genes,the physicochemical properties of proteins,and the tissue expression patterns,and also studied the responses to abiotic stresses.[Result]The total of eight GS genes were identified from the soybean,located on eight chromosomes,corresponding to coding amino acid sequences with lengths ranging from 356 to 432 aa.Among the ten conserved motifs,all eight GmGS contained nine conserved motifs,and GmGS7 and GmGS8 had one more motif 10 conserved motif than other members.Analysis of promoter's cis-acting elements showed that the promoters of GmGSs contained abundant light-responsive elements,hormone-responsive elements,and adversity stress-responsive elements.Transcriptome data analysis showed that GmGSs genes were expressed in all tissues,with GmGS3 and GmGS4 having higher expression in all tissues.Fluorescence quantitative qPCR results revealed that GmGS4,GmGS5,and GmGS7 of soybean GS family responded most significantly to the late stage of low concentration ammonium treatment after different concentrations of ammonium chloride.And after high salt stress treatment,the expression of GmGS5 decreased in the root,stem and leaf tissues;while the expression of GmGS7 increased in root and stem tissues.[Conclusion]There are eight GS members in soybean,of which GmGS7 is involved in response to both ammonium chloride treatment and salt stress.
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