Genome-wide Identification of Non-specific Phospholipase C Gene Family in Hordeum vulgare L.and Stress Expression Analysis at Seedling Stage
[Objective]Non-specific phospholipase C(NPC)is a type of phospholipase C,which plays an important role in plant growth and development and response to abiotic stresses.Identifying the NPC gene family members from the whole barley genome,and analyzing the expression characteristics of the related genes would provide a theoretical basis for the study of barley NPC gene function and genetic improvement.[Method]Based on the barley genome-wide data included in the EnsemblPlants database,bioinformatics methods were used to identify NPC gene family in barley and analyze their physicochemical properties,subcellular localization,phylogenetic relationship,gene structure,protein structural domains,and promoter cis-acting elements.Transcriptome data and RT-qPCR were used to analyse the expression characteristics of HvNPCs in different tissues as well as at seedling stage under low temperature,drought and salt stress.[Result]Five NPC members were identified in the barley genome,and the encoded proteins were structurally similar to NPC genes from other plants,all of which had a phosphoesterase structural domain;the sequence lengths of the HvNPC proteins ranged from 477 to 553 aa,the isoelectric points ranged from 5.22 to 8.83,and the molecular weights ranged from 53.12 to 61.33 kD.Subcellular localization showed that the HvNPC gene was localized in chloroplasts,membrane-intrinsic proteins,and cytoplasm.The HvNPC promoter region contained a large number of cis-acting elements associated with adversity stresses,and real-time PCR analysis confirmed that the HvNPC gene was induced to express by drought,high salt and low temperature.Among them,HvNPC2,HvNPC3 and HvNPC5 were induced to be expressed by low-temperature stress and drought stress;and HvNPC3 and HvNPC5 were induced to be expressed by salt stress.[Conclusion]A total of five HvNPC genes were identified in the barley genome,and members of the HvNPC genes were organ-specofc expression and different genes responded differently to abiotic stresses.
万方数据
维普
