Genome-wide Identification and Expression Analysis of the SRO Gene Family in Brassica juncea L.
[Objective]SRO gene family is a class of plant-specific transcription factors,which plays an important role in plant growth and development and stress response.The study of the SRO gene family in Brassica juncea L.provides a theoretical basis for analyzing the function of SRO genes in B.juncea L.and its genetic improvement.[Method]Bioinformatics was used to identify members of the SRO gene family in the genomes of two types of oilseed mustard and vegetable mustard,the software and database such as TBtools,MEGA,Cytoscape,NCBI,STRING,EggNOG were used for the analysis of physicochemical properties,sequence characterization,evolutionary relationships,regulatory networks,etc.,as well as RT-qPCR to analyze the expression patterns under salt stress.[Result]There were differences in the number of members of the SRO gene family between the two types of B.juncea L.,and they belonged to two major classes.Among them,class A SRO proteins contained WWE,PARP and RST structural domains,whereas class B proteins were lack of WWE domains.The promoter region of the SRO genes contained a variety of cis-acting elements for abiotic stress response and hormone response.The microRNA-BjuSRO-target genes constructed a complex regulatory network,which was involved in apoptosis,root morphogenesis,immune response,cellular response to heterologous stimuli,and response to reactive oxygen species,and other biological processes.The RT-qPCR results showed that BjuVA1a,BjuVA1e,BjuVA2a,BjuVA3a,BjuVB1b,and BjuVA2a were significantly upregulated under salt stress.[Conclusion]The SRO gene family in the mustard has functional diversity,and BjuVA1a,BjuVA1e,BjuVA2a,BjuVA3a,BjuVB1b,and BjuVA2a are closely related to the response to salt stress,which can be used as candidate genes for breeding new salt-tolerant varieties of B.juncea L.
万方数据
维普
