Identification of the bHLH Gene Family and Selection of Genes Related to Color Formation in Camellia reticulata
[Objective]The preliminary screening of bHLH genes related to C.reticulata flower color by bioinformatics method provides support for further study of C.reticulata flower color.[Method]Based on the full-length transcriptome data of C.reticulata,the members of the bHLH gene family were identified by bioinformatics method,and their physical and chemical properties,secondary structure,phylogenetic relationships and domains were analyzed.The second-generation transcriptome and metabolome data were used to mine the bHLH genes related to the flower color of C.reticulata.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to verify the expression of bHLH gene during flowering of C.reticulata among different varieties.[Result]Total 71 CrbHLHs genes were identified in the full-length transcriptome of C.reticulata.Their molecular weight ranged from 22 994.58 to 102 996.96 Da,and their equipotential ranged from 4.98 to 9.51.All of them were hydrophilic proteins.The cluster analysis with Arabidopsis thaliana showed that the bHLH gene family members of C.reticulata could be distributed into 14 subfamilies.Multiple omics analysis showed that CrbHLH8,CrbHLH61 and CrbHLH63 were positively correlated with cyanidin,while CrbHLH59 was negatively correlated with cyanidin.CrbHLH13 and CrbHLH71 were positively correlated with proanthocyanidins,and CrbHLH8 and CrbHLH61 were members of the IIIf subfamily.[Conclusion]The 71 CrbHLHs family members were identified from the full-length transcriptome of C.reticulata,of which 6 CrbHLHs were related to C.reticulata flower color.
万方数据
维普
