Genome-wide Identification and Expression Pattern Profiling of the Trehalose-6-phosphate Synthase(TPS)Gene Family in Tea Plant(Camellia sinensis)
[Objective]Trehalose-6-phosphate synthase(TPS)is a key enzyme in the trehalose biosynthetic pathway in plants.The TPS gene family of the tea plant[Camellia sinensis(L.)O.Kuntze]was identified,analyzed,and its expression pattern under adverse stress was studied.This study aims to provide a foundation for the functional validation of TPS genes.[Method]Utilizing biological information methods,we identified members of CsTPS gene family and analyzed the physical and chemical properties of their encoded protein,conserved motifs and domains,chromosomal location,phylogenetic relationships,promoter cis-acting elements,and other biological information.Subsequently,we employed transcriptome data in conjunction with RT-qPCR technology to investigate the expression patterns of CsTPS family members under drought,salt,and cold stress.[Result]The 16 TPS gene family members were identified in C.sinensis genome,which were classified into two subfamilies:Class I and Class II based on phylogenetic study.Members of the same subfamily presented similar motif composition and gene structure.The covariance and evolutionary studies revealed that CsTPS genes were duplicated within the tea plant genome and experienced purifying selection during the evolutionary process.RT-qPCR results revealed that the six candidate genes,CsTPS3,CsTPS5,CsTPS6,CsTPS9,CsTPS11,and CsTPS14,were all induced by PEG,NaCl,and low-temperature stress,and their expression patterns were similar with transcriptome information.[Conclusion]Sixteen TPS family members were identified systematically across the tea plant's genome.The CsTPS3,CsTPS5,CsTPS6,CsTPS9,CsTPS11,and CsTPS14 genes all are sensitive to drought,salt,and low-temperature stresses,though their sensitivity levels differed.
万方数据
维普
