Analysis of LncRNA Differential Expression in Mammary Tissue of Cows with Staphylococcus aureus Mastitis
[Objective]Long non-coding RNA(lncRNA)is involved in inflammatory responses,however,their specific molecular regulatory mechanism in dairy mastitis remains unclear.In this study,differentially expressed lncRNA(DElncRNA)and its function in mammary tissues of cows with Staphylococcus aureus(S.aureus)-type mastitis were analysed to support subsequent in-depth studies.[Method]Transcriptome sequencing(RNA-Seq)technology and bioinformatics methods were used to carry out lncRNA sequencing,DElncRNA analysis,and GO and KEGG functional enrichment analysis of mammary tissues from healthy cows and cows with S.aureus-induced mastitis.[Result]A total of 1012 lncRNA are detected in the mammary tissues of both groups.Compared to the healthy dairy cows,total 75 lncRNA are up-regulated and 114 down-regulated in cows with S.aureus-induced mastitis.Further analysis reveals that DElncRNA TCONS_00042123,TCONS_00068055,TCONS_00108420 and TCONS_00076361 may be involved in the MAPK,NLR,Jak-STAT and TLR signaling pathways related to inflammation,regulating the occurrence and development of S.aureus-type mastitis in the dairy cows.[Conclusion]189 DElncRNA were obtained from mammary tissues of cows with S.aureus-induced inflammation,which may regulate the process of onset and progression of S.aureus-type cow mastitis through potential target genes.
万方数据
维普
