首页|彩色马铃薯花青素合成相关ERF基因筛选及表达分析

彩色马铃薯花青素合成相关ERF基因筛选及表达分析

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
[目的]乙烯响应因子(ethylene responsive factor,ERF)是一类重要的转录因子,参与调控植物花青素的生物合成.筛选可能参与调控马铃薯块茎花青素合成的StERFs基因,为开展彩色马铃薯花青素相关StERFs基因功能研究奠定基础.[方法]利用生物信息学方法对其理化性质、亚细胞定位、保守基序、进化关系、蛋白质二级结构、启动子顺式作用元件及蛋白互作关系进行分析,同时采用RT-qPCR方法对不同颜色马铃薯薯肉中StERFs进行表达模式分析.[结果]基于不同颜色马铃薯块茎的转录组表达谱,获得 7 个差异表达ERF转录因子.7 个StERFs属于亲水性蛋白,均预测定位于细胞核.Motif 1(RWLG)和Motif 2(YRG)是 7 个StERFs中共同的保守基序,属于AP2 的结构域特征序列.StERFs基因启动子序列含有响应激素作用元件,以及响应防御与胁迫、低温和光照等的顺式作用元件.7 个StERFs在紫色薯肉中表达量显著高于黄色薯肉,其中StERF72 和StERF110 与已知的花青素相关ERFs(IbERF71 和PyERF3)亲缘关系较近,且 7 个StERFs与 56 个转录因子存在蛋白互作关系.[结论]7 个StERFs基因可能参与调控马铃薯块茎中花青素的合成,其中StERF72和StERF110可作为候选基因进一步验证其功能.
Screening and Expression Analysis of ERF Gene Related to Anthocyanin Synthesis in Colored Potato
[Objective]Ethylene responsive factor(ERF)is an important transcription factor involved in the regulation of anthocyanin biosynthesis in plants.Screening of StERF genes that may be involved in the regulation of anthocyanin synthesis in potato tubers lays the foundation for the next step of research on the function of anthocyanin-related StERFs genes in colored potato.[Method]Bioinformatics methods were used to analyze their physicochemical properties,subcellular localization,conserved motifs,evolutionary relationships,protein secondary structure,promoter cis-acting elements,and protein interactions,while RT-qPCR was used to analyze the expression patterns of StERFs in potato flesh of different colors.[Result]Seven differentially expressed ERF transcription factors were obtained based on the transcriptome expression profiles of potato tubers of different colors.The seven StERFs are hydrophilic proteins,all of which are predicted to be localized in the nucleus.Motif 1(RWLG)and Motif 2(YRG)are the common conserved motifs in the seven StERFs,which belong to the structural domains of the characteristic sequences of AP2.The promoter sequences of the StERFs genes contain hormone-responsive action elements,as well as cis-action elements in response to defense and stress,low temperature and light,etc.The expressions of the seven StERFs were significantly higher in purple potato flesh than in yellow potato flesh,among which StERF72 and StERF110 were closer to known anthocyanin-associated ERFs(IbERF71 and PyERF3).The existence of protein interactions between the seven StERFs and 56 transcription factors.[Conclusion]Seven StERFs may be involved in the regulation of anthocyanin synthesis in potato tubers,among which StERF72 and StERF110 can be candidated genes to further validate their functions.

colored potatoanthocyaninERF transcription factorbioinformatics analysisexpression pattern analysis

吴娟、武小娟、王沛捷、谢锐、聂虎帅、李楠、马艳红

展开 >

内蒙古农业大学农学院,呼和浩特 010019

内蒙古自治区农牧业科学院,呼和浩特 010010

彩色马铃薯 花青素 ERF转录因子 生物信息学分析 表达模式分析

内蒙古自治区马铃薯育种联合攻关项目内蒙古农业大学基本科研业务费专项内蒙古自治区研究生科研创新项目内蒙古自治区高校基础科研专项基金项目(2022)

YZ2023006BR22-11-16B20210182Z

2024

10.13560/j.cnki.biotech.bull.1985.2024-0460

生物技术通报
中国农业科学院农业信息研究所

生物技术通报

CSTPCD北大核心
影响因子：0.505
ISSN：1002-5464
年,卷(期)：2024.40(9)