基于SSR荧光标记毛细管电泳的马铃薯品种遗传多样性分析及分子身份证构建
Genetic Diversity Analysis of Potato Varieties Based on SSR Fluorescent Marker Capillary Electrophoresis and Construction of Molecular Identity Card
毛向红 1卢瑶 1范向斌 1杜培兵 1白小东1
作者信息
- 1. 山西农业大学高寒区作物研究所 马铃薯遗传改良与种质创新山西省重点实验室,大同 037008
- 折叠
摘要
[目的]对引进的马铃薯品种进行遗传多样性分析和评价.[方法]使用SSR荧光标记毛细管电泳法分析 51 份引进的马铃薯品种的遗传多样性,并构建分子身份证.[结果]41 对引物共扩增出 327 个等位位点,311 个等位位点在相同位置存在差异性,多态性比例是 95.11%;引物多态性信息指数(PIC)在 0-0.89 之间,平均为 0.71;Shannon's(Ⅰ)在 0-0.54 之间,平均为 0.32.在遗传相似系数GS为 0.76 时,将供试材料分为 6 大类,第I类包括 14 个品种;第Ⅱ类包括 30 个品种,第Ⅲ类包括 3 个品种,第V类包括 2 个品种;第Ⅳ类和第Ⅵ类各包含 1 个品种.聚类分析发现维拉斯、定薯 4 号、克新 33 号和东农 312 这 4 个品种可作为马铃薯育种材料.[结论]筛选到 3 对核心引物STM0037、SSR0387、SSR01054 可用于构建 51 个马铃薯品种的指纹图谱和分子身份证.
Abstract
[Objective]Genetic diversity analysis and evaluation of introduced potato varieties is a necessary method for screening good parental materials.[Method]SSR fluorescent marker capillary electrophoresis was used to analyze the genetic diversity of 51 introduced potato varieties and construct molecular ID cards.[Result]The 41 pairs of primers amplified a total of 327 alleles,and 311 alleles were differentiated at the same position,and the percentage of polymorphism was 95.11%.The primer polymorphism information index(PIC)ranged from 0 to 0.89,with an average of 0.71.Shannon's(I)ranged from 0 to 0.54,with an average of 0.32.The genetic similarity coefficient(GS)of the introduced potato varieties was 0.32.In the genetic similarity coefficient GS of 0.76,the test materials were categorized into six major groups:Group Ⅰ having 14 varieties,group Ⅱ having 30 varieties,group Ⅲ having three varieties,group V having two varieties,and group Ⅳ and Ⅵ having one variety each.Cluster analysis revealed that four varieties,Vilas,Dingshu 4,Kexin 33 and Dongnong 312 could be used as potato breeding materials.[Conclusion]Three pairs of core primers,STM0037,SSR0387 and SSR01054,were selected to construct fingerprints and molecular IDs of 51 potato varieties.
关键词
马铃薯/SSR/毛细管电泳/遗传多样性/分子身份证Key words
potato/SSR/capillary electrophoresis/genetic diversity/molecular identity card引用本文复制引用
基金项目
国家马铃薯产业技术体系(CARS-09-ES02)
山西省重点研发计划项目(202102140601004)
山西农业大学生物育种工程(YZGC082)
山西省现代农业产业技术体系建设专项资金资助(2024CYJSTX06)
出版年
2024
国家科技期刊平台
NSTL
万方数据