Genetic Diversity Analysis of Potato Varieties Based on SSR Fluorescent Marker Capillary Electrophoresis and Construction of Molecular Identity Card
[Objective]Genetic diversity analysis and evaluation of introduced potato varieties is a necessary method for screening good parental materials.[Method]SSR fluorescent marker capillary electrophoresis was used to analyze the genetic diversity of 51 introduced potato varieties and construct molecular ID cards.[Result]The 41 pairs of primers amplified a total of 327 alleles,and 311 alleles were differentiated at the same position,and the percentage of polymorphism was 95.11%.The primer polymorphism information index(PIC)ranged from 0 to 0.89,with an average of 0.71.Shannon's(I)ranged from 0 to 0.54,with an average of 0.32.The genetic similarity coefficient(GS)of the introduced potato varieties was 0.32.In the genetic similarity coefficient GS of 0.76,the test materials were categorized into six major groups:Group Ⅰ having 14 varieties,group Ⅱ having 30 varieties,group Ⅲ having three varieties,group V having two varieties,and group Ⅳ and Ⅵ having one variety each.Cluster analysis revealed that four varieties,Vilas,Dingshu 4,Kexin 33 and Dongnong 312 could be used as potato breeding materials.[Conclusion]Three pairs of core primers,STM0037,SSR0387 and SSR01054,were selected to construct fingerprints and molecular IDs of 51 potato varieties.
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