基于根表真菌群落与病原菌鉴定探究'宁杞5号'枸杞根腐病的发生机制
Exploring on the Pathogenesis of Root Rot of Lycium barbarum cv.'Ningqi-5'Based on the Rhizoplane Fungal Community and Pathogens Identification
张亚亚 1李盼盼 1高惠惠 1贾晨波 1徐春燕1
作者信息
- 1. 宁夏大学生命科学学院 西部特色生物资源保护与利用教育部重点实验室,银川 750021
- 折叠
摘要
[目的]比较两种植区易感病品种'宁杞 5 号'枸杞健康株和患病株的根表真菌群落组成和分离到的腐根真菌,明确'宁杞 5 号'根腐病的病原菌,探究两种植区'宁杞 5 号'根腐病的发生原因.[方法]采用高通量测序技术研究枸杞根表真菌群落的组成特征,采用组织分离法从两种植区枸杞腐根中分离腐根真菌,基于形态学和ITS、EF-1α基因序列对腐根真菌进行分类学鉴定,依据科赫法则进行致病性研究.[结果]两种植区'宁杞 5 号'枸杞健康株和患病株之间的根表真菌群落组成均表现出明显差异,主要体现在患病后镰刀菌属(Fusarium)相对丰度升高,被孢霉属(Mortierella)相对丰度降低,伴随着Fusicolla和Pseudogymnoascus等未知类群丰度增加,而两种植区健康株根表真菌群落结构趋于一致.自QXBZ腐根分离到 36 株真菌,分别鉴定为尖孢镰刀菌(Fusarium oxysporum)、腐皮镰刀菌(F.solani)、红贝俄式孔菌(Earliella scabrosa)、立枯丝核菌(Rhizoctonia solani)和Penicillium pimiteouiense,自QTBZ腐根分离 29 株真菌,分别鉴定为尖孢镰刀菌、腐皮镰刀菌、红贝俄式孔菌、粉红黏帚菌(Clonostachys rosea)、P.pimiteouiense、新知镰刀菌(F.andiyazi)、桃色顶孢霉(Acremonium persicinum),其中腐皮镰刀菌的分离频率在两种植区均为最高,尖孢镰刀菌次之.回接实验确定腐皮镰刀菌、尖孢镰刀菌、新知镰刀菌和立枯丝核菌是'宁杞 5 号'枸杞根腐病的病原菌,其中新知镰刀菌为新发现的枸杞根腐病病原菌.[结论]'宁杞 5 号'根腐病发生主要与根表真菌群落结构改变以及病原菌种类密切相关,与种植区关系不大,F.solani、F.oxysporum、F.andiyazi和R.solani是'宁杞 5 号'根腐病的病原菌.
Abstract
[Objective]This paper is to compare the composition of rhizoplane fungal communities in susceptible strain'Ningqi-5'between the healthy and diseased plants in the two planting areas(QXBZ and QTBZ),as well as the isolated rot root fungi.It is also to clarify the pathogens causing root rot disease in'Ningqi-5',and to explore the reasons for the root rot disease in'Ningqi-5'.[Method]High-throughput technology was applied to investigate the compositional characteristics of the rhizoplane fungal community.Tissue isolation method was to isolate the rot-root fungi the decayed roots of Lycium barbarum.They were taxonomically identified based on the morphological characteristics and the sequences of ITS and EF-1α gene fragments.The pathogenicity bythe rotten-root fungi was verified based on Koch's law.[Result]The root rhizoplane fungal community composition differed significantly between the healthy and diseased plants of'Ningqi-5'in both planting areas,showing the increase in relative abundance of Fusarium and decrease in relative abundance of Mortierella in the diseased plants,accompanying with the increase of unknown taxa like Fusicolla and Pseudogymnoascus.While the fungal community structure on the rhizoplane of the healthy plants in both planting areas tended to be consistent.The 36 strains isolated from the rotten rot of QXBZ were classified as Fusarium oxysporum,F.solani,Earliella scabrosa,Rhizoctonia solani,and Penicillium pimiteouiense.The 29 strains isolated from the rotten rot of QTBZ were classified as F.oxysporum,F.solani,E.scabrosa,Clonostachys rosea,P.pimiteouiense,F.andiyazi and Acremonium persicinum.Isolation frequency of F.solani was the highest in both planting areas,followed by F.oxysporum.Based on the artificial infection test,F.solani,F.oxysporum,R.solani,and F.andiyazi were identified as the pathogens of'Ningqi-5'root rot disease,among which F.andiyazi was a newly discovered pathogen of L.barbarum root rot.[Conclusion]In this study,it is clarified that the occurrence of'Ningqi-5'root rot disease is mainly closely related to the changes in the structure of the root rhizoplane fungal community and pathogens.F.solani,F.oxysporum,F.andiyazi,and R.Solani are the pathogens of'Ningqi-5'root rot disease.
关键词
枸杞/根表真菌群落/根腐病/病原菌/分离鉴定Key words
Lycium barbarum/rhizoplane fungal community/root rot disease/pathogen/isolation and identification引用本文复制引用
基金项目
宁夏自然科学基金项目(2020AAC02010)
出版年
2024
国家科技期刊平台
NSTL
万方数据