多粘类芽胞杆菌新菌株X-11及其对番茄和水稻的促生效应
Paenibacillus polymyxa New Strain X-11 and Its Growth-promoting Effects on Tomato and Rice
刘文志 1贺丹 2李鹏 2傅应林 1张译心 1温华杰 1于文清3
作者信息
- 1. 上饶师范学院生命科学学院,上饶 334001;上饶师范学院生态环境与绿色发展研究中心 赣东北特色植物资源保育与生态修复重点实验室,上饶 334001
- 2. 黑龙江省农垦科学院,哈尔滨 150038
- 3. 上饶师范学院生命科学学院,上饶 334001;上饶师范学院生态环境与绿色发展研究中心 赣东北特色植物资源保育与生态修复重点实验室,上饶 334001;黑龙江省农垦科学院,哈尔滨 150038
- 折叠
摘要
[目的]从小麦(Triticum aestivum L)根际筛选到了一株细菌菌株(编号X-11),为该菌株在植物促生上应用提供依据.[方法]结合形态特征、生理生化特性、16S rDNA序列及基因组测序对其进行了鉴定,并测定其对番茄(Solanum lycopersicum)和水稻(Oryza sativa)的促生效应.[结果]该菌株菌落圆形、半透明、凸起、黏稠;菌体杆状,形成芽胞,革兰氏阴性;具有固氮,还原硝酸盐,产吲哚、分泌铁载体及分解有机磷的功能.基于 16S rDNA序列比对将X-11 菌株归类为多粘类芽胞杆菌种群,基于非冗余蛋白数据库的基因比对表明其与最近缘菌株基因相似率 55.7%,证明其为一个新菌株.X-11 菌液浸种显著增加了番茄发芽率和胚根伸长度,灌根(R)及灌根结合叶面喷施(R+L)显著提高了番茄叶片中过氧化物酶(POD)活性,R+L处理使超氧化物歧化酶(SOD)活性提高了 34.58%.水稻 1 叶 1 心期施用X-11 菌液,根长增加了 6.8%;立针期或 1 叶 1 心期施用X-11 菌液,使秧苗SOD活性分别提高了 90.4%和 51.8%.[结论]X-11 被鉴定为多粘类芽胞杆菌新菌株,是多种功能的植物根际促生菌,在植物促生方面具有开发应用潜力.
Abstract
[Objective]A plant-growth-promoting rhizobacterium strain(named X-11)was screened from rhizosphere soil of wheat(Triticum aestivum L).Which may provide a basis for the application of the strain in plant growth-promoting.[Method]Strain X-11 was identified by morphological characteristics,physiological and biochemical characteristics,16S rDNA sequence and genome sequencing,and its effects on the growth and defense enzyme activities of tomato(Solanum lycopersicum)and rice(Oryza sativa)were measured.[Result]The colonies of strain X-11 are round,swell,neat edge,translucent,and sticky.And the cells are rod-shaped,can form spores,gram negative reaction.And it has the functions of fixing nitrogen,reducing nitrate,producing indole and siderophore,degrading organic phosphorus.Strain X-11 was classified as Paenibacillus polymyxa group based on 16S rDNA sequence,while it was revealed to be a new strain with 55.7%genetic similarity to its closest relative strain through gene comparison in non-redundant database.Tomato(Solanum lycopersicum)seeds soaked with X-11 bacterial suspension increased germination rate and radicle length significantly.Moreover,peroxidase(POD)activity in the leaves significantly increased by X-11 bacterial suspension irrigating roots(R)and irrigating roots combined with spraying leaf surface(R+L).Meanwhile,the R+L treatment increased superoxide dismutase(SOD)activity by 34.58%.X-11 bacterial suspension increased the seedling root length by 6.8%when applied at one-leaf phase of rice(Oryza sativa).Additionally,the SOD activity of the seedlings increased by 90.4%and 51.8%,when the X-11 bacterial suspension was applied at vertical phase or one-leaf phase of rice.[Conclusion]Strain X-11 was identified as a new strain of P.polymyxa,which is the effective plant-growth promoting rhizobacterium with multiple functions and has potential for development and application in plant growth promotion.
关键词
多粘类芽胞杆菌/植物根际促生菌/番茄/水稻Key words
Paenibacillus polymyxa/plant-growth promoting rhizobacterium/tomato/rice引用本文复制引用
基金项目
国家自然科学基金项目(32360698)
国家自然科学基金项目(32060633)
黑龙江省自然科学基金项目(LH2021C087)
高标准农田建设和黑龙江垦区社会公益性事务专项(KJZX202204-11)
出版年
2024
国家科技期刊平台
NSTL
万方数据