Paenibacillus polymyxa New Strain X-11 and Its Growth-promoting Effects on Tomato and Rice
[Objective]A plant-growth-promoting rhizobacterium strain(named X-11)was screened from rhizosphere soil of wheat(Triticum aestivum L).Which may provide a basis for the application of the strain in plant growth-promoting.[Method]Strain X-11 was identified by morphological characteristics,physiological and biochemical characteristics,16S rDNA sequence and genome sequencing,and its effects on the growth and defense enzyme activities of tomato(Solanum lycopersicum)and rice(Oryza sativa)were measured.[Result]The colonies of strain X-11 are round,swell,neat edge,translucent,and sticky.And the cells are rod-shaped,can form spores,gram negative reaction.And it has the functions of fixing nitrogen,reducing nitrate,producing indole and siderophore,degrading organic phosphorus.Strain X-11 was classified as Paenibacillus polymyxa group based on 16S rDNA sequence,while it was revealed to be a new strain with 55.7%genetic similarity to its closest relative strain through gene comparison in non-redundant database.Tomato(Solanum lycopersicum)seeds soaked with X-11 bacterial suspension increased germination rate and radicle length significantly.Moreover,peroxidase(POD)activity in the leaves significantly increased by X-11 bacterial suspension irrigating roots(R)and irrigating roots combined with spraying leaf surface(R+L).Meanwhile,the R+L treatment increased superoxide dismutase(SOD)activity by 34.58%.X-11 bacterial suspension increased the seedling root length by 6.8%when applied at one-leaf phase of rice(Oryza sativa).Additionally,the SOD activity of the seedlings increased by 90.4%and 51.8%,when the X-11 bacterial suspension was applied at vertical phase or one-leaf phase of rice.[Conclusion]Strain X-11 was identified as a new strain of P.polymyxa,which is the effective plant-growth promoting rhizobacterium with multiple functions and has potential for development and application in plant growth promotion.
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