蛹虫草提取物对高尿酸血症大鼠的降尿酸作用及机制
Effect and Mechanism of Cordyceps militaris Extract on Lowering Uric Acid in Hyperuricemia Rats
熊心怡 1刘利平 2冯杰 2张劲松 2李德顺 2刘朋 2刘艳芳2
作者信息
- 1. 上海海洋大学食品学院,上海 201306;上海市农业科学院食用菌研究所 农业农村部南方食用菌资源利用重点实验室 国家食用菌工程技术研究中心,上海 201403
- 2. 上海市农业科学院食用菌研究所 农业农村部南方食用菌资源利用重点实验室 国家食用菌工程技术研究中心,上海 201403
- 折叠
摘要
[目的]探究蛹虫草(Cordyceps militaris)提取物对高尿酸血症模型大鼠的降尿酸(uric acid,UA)作用及其机制.[方法]测定蛹虫草提取物总糖、还原糖、多糖、蛋白质和多酚物质含量,基于灌胃氧嗪酸钾联合酵母膏饲养建立高尿酸血症大鼠模型,通过对大鼠的血清UA水平、肾功能、氧化应激和炎症以及肠道微生物组成变化等测定以评价蛹虫草提取物对高尿酸血症大鼠的影响作用及其机制.[结果]蛹虫草提取物含有 35.86%多糖、27.05%蛋白质及 0.21%的酚类物质.动物实验结果表明,蛹虫草提取物降低高尿酸血症大鼠血清的UA水平,其中 0.5 g/(kg·d)的蛹虫草提取物效果最佳,可使UA水平从 281.62 μmol/L降至 93.27 μmol/L,抑制率为 66.88%,同时可抑制高尿酸血症大鼠肾脏组织的氧化应激和炎症反应.分子机制研究表明,蛹虫草提取物可以下调高尿酸血症大鼠肾脏组织中尿酸盐阴离子转运蛋白(uric acid transporter 1,URAT1)、葡萄糖转运蛋白 9(glucose transporter 9,GLUT9)的表达水平;上调有机阴离子转运蛋白 1(organic anion transporter 1,OAT1)和三磷酸腺苷结合盒转运蛋白G2(ATP binding cassette subfamily G member 2,ABCG2)的表达水平.蛹虫草提取物能显著抑制肝脏组织中的黄嘌呤氧化酶活力.此外,蛹虫草提取物能够提升高尿酸血症大鼠肠道微生物的多样性,维持其肠道微生态平衡.[结论]蛹虫草提取物具有良好的抗高尿酸血症的作用,可能与调节尿酸转运蛋白表达水平和抑制黄嘌呤氧化酶活力等有关.
Abstract
[Objective]To investigate the effect and the corresponding mechanism of Cordyceps militaris(C.militaris)extract on lowering uric acid by hyperuricemia model rats.[Method]The contents of total sugars,reducing sugars,polysaccharides,proteins and polyphenols in C.militaris extract were determined.The rat model of hyperuricemia was established based on feeding of potassium oxonate combined with yeast paste.The serum uric acid level,renal function,oxidative stress and inflammation of rats and the changes of intestinal microbial composition were measured to evaluate the effect of C.militaris extract on hyperuricemia rats and its mechanism.[Result]The C.militaris extract contained 35.86%polysaccharides,27.05%proteins,and 0.21%polyphenols.Animal experiments demonstrated that C.militaris extract significantly reduced the serum uric acid levels in hyperuricemia rats.The most effective dose was found to be 0.5 g/(kg·d),leading to a reduction in uric acid levels from 281.62 μmol/L to 93.27 μmol/L,with an inhibition rate of 66.88%.It also inhibited the oxidative stress and inflammation in renal tissue of hyperuricemia rats.Molecular mechanism studies showed that C.militaris extract down-regulated uric acid transporter1(URAT1)and glucose transporter 9(GLUT9),up-regulated the expressions of organic anion transporter 1(OAT1)and ATP binding cassette subfamily G member 2(ABCG2)in renal tissues of hyperuricemia rats.Concurrently,C.militaris extract significantly inhibited the xanthine oxidase activity in liver tissues.In addition,C.militaris extract enhanced the diversity of intestinal microbiota and maintained the balance of intestinal microecology in hyperuricemia rats.[Conclusion]C.militaris extract demonstrated a good anti-hyperuricemia effect,which might be related to regulating the expression of uric acid transporter and inhibiting the activity of xanthine oxidase.
关键词
高尿酸血症/蛹虫草/水提物/尿酸转运蛋白/肠道微生物Key words
hyperuricemia/Cordyceps militaris/water extract/uric acid transporters/gut microbiota引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据