Effect of Cultivation Conditions on the Content of Polysaccharide in Ganoderma tsugae and its Antioxidant Activity
[Objective]The aim of this study is to optimize the cultivation and polysaccharide extraction conditions of Ganoderma tsugae,analyze its antioxidant activity in vitro,and clarify the polysaccharide activity of G.tsugae.[Method]The agronomic traits and sugar content of G.tsugae were used as indicators to optimize the cultivation conditions and analyze its antioxidant activity in vitro.Furthermore,response surface design was used to optimize the extraction of G.tsugae crude polysaccharide(GTP)and to investigate its protective effect on H2O2 induced HepG2 cells.[Result]The fruiting body mass and polysaccharide content are the highest,while the optimal cultivating conditions for G.tsugae is shading rate of 60%,temperature of 30℃,humidity of 80%,and CO2 concentration of 0.03%.Water extracts from G.tsugae also present promising antioxidant activity in vitro,with the ability to scavenge free radicals and resist superoxide anions.The optimal extraction method for sugar is at an extraction temperature of 82℃,extraction time of 5.6 h,and a liquid-solid ratio of 56 mL/g,reaching 1.90%.[Conclusion]The cultivation conditions and polysaccharide extraction process of G.tsugae were clarified,and it was confirmed that it has antioxidant activity in vitro.Its protective effect on H2O2-induced HepG2 is related to oxidative stress.
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