In Vitro Anti-tumor Effect and Its Mechanism of Polyphenols from Sanghuangporus vaninii Based on Network Pharmacology
[Objective]The polyphenolic components with excellent anti-cancer activity from the fruiting body of Sanghuangporus vaninii were isolated and screened,and their anti-cancer activity and potential mechanisms of action were analyzed.[Method]Ultrasound-assisted extraction was used to extract components from the ethyl acetate fraction.Polyphenolic components with excellent anti-cancer effects were screened for their inhibitory activity on liver cancer cell HepG-2.The main components were identified using ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)technology,and the potential mechanism of anti-cancer was elucidated using network pharmacology strategies.[Result]Five polyphenolic components(SVa-SVe)were isolated and prepared,among which SVe polyphenolic component demonstrated unique anti-cancer activity,with an inhibition rate of 76.54%on HepG-2 cells at a concentration of 100 μg/mL.SVe significantly promoted apoptosis and induced cell cycle arrest in HepG-2 cells,showing a dose-dependent effect.Ingredient analysis showed that SVe was mainly composed of 31 compounds.Based on network pharmacology analysis,key compounds such as osmundacetone,hispolo,phellibaumin A,davallialactone in SVe exerted anti-cancer activity by interacting with multiple target proteins(STAT3,mTOR,VEGFA,SRC,ERBB2,and HSP90AA).[Conclusion]The extract SVe derived from S.vaninii possessed unique inhibitory activity on HepG-2 cells by inducing apoptosis and cell cycle arrest,which was related to the presence of polyphenolic substances.
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