Study on the Optimization of Ultrasonic-assisted Extraction Process of Hericium coralloides(Scop.)Pers.Polysaccharides and Its Anti-colorectal Cancer Activity
[Objective]Hericium coralloides(Scop.)Pers.is a medicinal and edible fungus.This study focused on optimizing the ultrasonic extraction process of its polysaccharides(HCP)and exploring the in vitro anti-colorectal cancer activity and underlying mechanisms of HCP.[Method]Single-factor experiments combined with response surface methodology(RSM)S was used to optimize the ultrasonic extraction conditions of HCP.MTT assays and Western blot analyses were applied to investigate the anti-colorectal cancer activity and mechanisms of HCP.[Result]The optimal ultrasonic extraction conditions for H.coralloides polysaccharides were as follows:extraction power of 417 W,liquid-to-material ratio of 62 mL/g,and extraction time of 52.5 min.The predicted extraction yield of polysaccharides was 19.61%,with an actual yield of(19.58±0.05)%.HCP inhibited the growth of SW480 cells by reducing the phosphorylation levels of IKK,IκBα,and NF-κB,thereby decreasing the secretion of the pro-inflammatory factor IL-6.The half-maximal inhibitory concentration(IC50)was 1.246 mg/mL.[Conclusion]The optimal ultrasonic-assisted extraction rate of H.coralloides polysaccharides was 19.58%.HCP suppressed the secretion of pro-inflammatory cytokines by inhibiting the NF-κB signaling pathway,thus inhibiting the growth of SW480 cells.These findings suggest that HCP has potential therapeutic effects on colorectal cancer.
Hericium coralloidesanti-colorectal cancer activityultrasonic extractionresponse surface optimizationpolysaccharidesNF-κB signaling pathway
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