珊瑚状猴头菌多糖超声辅助提取工艺优化及抗结直肠癌活性研究
Study on the Optimization of Ultrasonic-assisted Extraction Process of Hericium coralloides(Scop.)Pers.Polysaccharides and Its Anti-colorectal Cancer Activity
余金岂 1邬柯奕 1陈正睿 1王婧涵 1张鑫 2王春月3
作者信息
- 1. 吉林农业大学植物保护学院,长春 130118
- 2. 吉林农业大学食药用菌教育部工程研究中心,长春 130118;沈阳农业大学园艺学院,沈阳 110866
- 3. 吉林农业大学植物保护学院,长春 130118;吉林农业大学食药用菌教育部工程研究中心,长春 130118
- 折叠
摘要
[目的]珊瑚状猴头菌(Hericium coralloides(Scop.)Pers.)是一种食药用菌,重点优化其多糖(HCP)的超声提取方案,并对HCP的体外抗结直肠癌活性及机制进行探索.[方法]利用单因素实验结合响应面法(response surface methodology,RSM)对HCP超声提取条件进行优化.通过MTT实验与Western blot分析对HCP的抗结直肠癌活性与机制进行研究.[结果]珊瑚状猴头菌多糖超声提取的最优提取方案如下:提取功率 417 W,液料比 62 mL/g,提取时间 52.5 min,多糖的提取率预测值为 19.61%,实际提取率为(19.58±0.05)%.HCP通过降低SW480 细胞中IKK、IκBα和NF-κB的磷酸化水平,减少了促炎因子IL-6 的分泌,抑制SW480 细胞生长,半数抑制浓度(half maximal inhibitory concentration,IC50)为 1.246 mg/mL.[结论]珊瑚状猴头菌多糖的最佳超声辅助提取率为 19.58%,HCP通过抑制NF-κB信号通路减少促炎细胞因子的分泌,进而抑制SW480 细胞生长,对于结直肠癌具有潜在的治疗作用.
Abstract
[Objective]Hericium coralloides(Scop.)Pers.is a medicinal and edible fungus.This study focused on optimizing the ultrasonic extraction process of its polysaccharides(HCP)and exploring the in vitro anti-colorectal cancer activity and underlying mechanisms of HCP.[Method]Single-factor experiments combined with response surface methodology(RSM)S was used to optimize the ultrasonic extraction conditions of HCP.MTT assays and Western blot analyses were applied to investigate the anti-colorectal cancer activity and mechanisms of HCP.[Result]The optimal ultrasonic extraction conditions for H.coralloides polysaccharides were as follows:extraction power of 417 W,liquid-to-material ratio of 62 mL/g,and extraction time of 52.5 min.The predicted extraction yield of polysaccharides was 19.61%,with an actual yield of(19.58±0.05)%.HCP inhibited the growth of SW480 cells by reducing the phosphorylation levels of IKK,IκBα,and NF-κB,thereby decreasing the secretion of the pro-inflammatory factor IL-6.The half-maximal inhibitory concentration(IC50)was 1.246 mg/mL.[Conclusion]The optimal ultrasonic-assisted extraction rate of H.coralloides polysaccharides was 19.58%.HCP suppressed the secretion of pro-inflammatory cytokines by inhibiting the NF-κB signaling pathway,thus inhibiting the growth of SW480 cells.These findings suggest that HCP has potential therapeutic effects on colorectal cancer.
关键词
珊瑚状猴头菌/抗结直肠癌活性/超声提取/响应面法/多糖/NF-κB信号通路Key words
Hericium coralloides/anti-colorectal cancer activity/ultrasonic extraction/response surface optimization/polysaccharides/NF-κB signaling pathway引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据