Effects of Bacillus amyloliquefaciens YK3 on the Control of Citrus reticulata cv.Orah Canker and Its Influence on the Network of Phyllosphere Bacteria
[Objective]To study the effects of Bacillus amyloliquefaciens YK3 on the control of Orah canker and its influence on the network of phyllosphere bacteria may provide practical reference for microbial prevention and control of Orah canker.[Method]The flat plate antibacterial method was used to isolate the antagonistic bacteria against the Orah canker.In vitro bioassay and potted experiments were used to study the control effect of antagonistic bacteria on Orah canker.The effects of inoculated biocontrol bacteria on bacterial community composition,abundance and bacterial interaction network were studied by high-throughput sequencing technology.[Result]A strain of Bacillus amyloliquefaciens YK3 was isolated,which had strong inhibitory effect on pathogenic bacteria.This strain produced bacteriostatic substances only when organic nitrogen was used as a nitrogen source and could not be used together with copper pesticides.The results of biocontrol test showed that the incidence rate of inoculation with pathogen alone was 96.67%,the incidence rate of inoculation with pathogen and YK3 was 33.33%.The control effect of YK3 on Orah canker was 65.56%.YK3 reduced the relative abundance of phyllosphere Xanthomonas.The positive correlation of phyllosphere bacterial community inoculated with only YK3 was the most complex,followed by simultaneous inoculation of pathogenic bacteria and YK3.The positive correlation of phyllosphere bacterial community inoculated with pathogenic bacteria was the weakest.Simultaneously inoculated with pathogens and YK3,Xanthomonas and Bacillus were negatively correlated with the indigenous bacterial community in phyllosphere.However,there was no overlap between Xanthomonas and Bacillus and the indigenous bacterial community in phyllosphere.Xanthomonas and Bacillus faced competition from different indigenous bacteria alone.[Conclusion]Bacillus amyloliquefaciens YK3 has a good control effect on Orah canker and can change the diversity and structure of phyllosphere bacteria in Orah.YK3 enhances the positive correlation network,weakens the negative correlation network and improved the interaction network.
Citrus reticulata cv.Orahcankerbiological controlBacillus amyloliquefaciensantagonistic effectphyllospherebacterial community diversitynetwork interaction
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