Effect of Forsythia suspensa Leaf Tea on with Cirrhosis and Its Mechanism in Rats
[Objective]To study the effects of crude extracts of Forsythia suspensa leaves and their tea solution on carbon tetrachloride(CCl4)induced liver cirrhosis in rats,and to elucidate the effects and mechanisms of different doses of the crude extracts and tea solution of F.suspensa leaves on gut microbiota diversity.[Method]The 30 male SD rats were randomly divided into control group,model group,low,medium,and high-dose F.suspensa leaf tea orally administered group,and tea water group according to the principle of similar average body weight.Liver cirrhosis rat model was constructed by subcutaneous injection of CCl4+CCl4 olive oil mixture orally administered group.At the same time,different doses of F.suspensa leaf tea crude extract orally administered,and tea water group were intervened for 10 d.Serum was collected for ELISA detection,liver tissue was stained with HE,the liver tissue RNA was extracted for RT-PCR detection,and fecal samples were subjected to 16S rRNA sequencing.[Result]Both the crude extract of F.suspensa leaves and the tea group significantly improved the morphology of liver cirrhosis in rats,especially the tea group significantly reversed the status of liver cirrhosis.Liver cell steatosis,inflammatory cell infiltration around the central vein,and fibrous connective tissue proliferation in the portal area were significantly improved compared to the model group.The crude extract of F.suspensa leaves and the tea group significantly increased the abundance of probiotics Rothia,Paraacteroides,and Lactobacillus,promoting the synthesis of unsaturated fatty acids and the enrichment of acid respiration pathways.The crude extract of F.suspensa leaf tea and the tea group inhibited the levels of DAO,TLR-4,and LPS endotoxin factors in the blood,promoted the expression of anti-inflammatory factor 1L-4 and intestinal barrier function factor ZO-1.The composition of intestinal microbiota among the groups was correlated with DAO,LPS,1L-4,and TLR4,with the highest correlation with intestinal injury index DAO.[Conclusion]The crude extract of F.suspensa leaf tea and tea group inhibit in the pathogenesis of liver cirrhosis by improving the pathological morphology of liver cirrhosis in rats,reducing inflammatory response,improving intestinal microecological dysfunction,and protecting intestinal barrier function.
Forsythia suspensa leaf tealiver cirrhosisdiversity of microbesratinflammatory factorsmechanism
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