Variation of Bioactivities and Secondary Metabolomics of Marine Fungus Aspergillus unguis DLEP2008001 Cultured under Different Salinities
[Objective]A marine fungus Aspergillus unguis DLEP2008001 was used for investigating the influence of salinity on the bioactivity and secondary metabolites.[Method]The crude extract samples of this strain cultured under different salinities in solid and liquid mediums for 14 and 28 days were analyzed by thin layer chromatography and antioxidative,acetylcholinesterase(AChE)inhibitory,and antimicrobial bioautographies to compare the bioactive secondary metabolites.The secondary metabolite diversity was further investigated by high performance liquid chromatography and"Multi-approach Assisted Feature Based Molecular Network(FBMN)"metabolomic analytical method based on liquid chromatography-tandem mass spectrometry(LC-MS/MS).[Result]The salinity regulated the yield and diversity of antioxidant,AChE inhibitory ingredients and anti-microbial.In seawater-potato-sucrose liquid medium,the strain presented the most fertile feature metabolites when cultured for 28 d under salinity of 35 g/L,while in rice solid medium,it produced the most abundant predominant compounds under salinity of 5 g/L and 35 g/L as the second best condition.The average molecular weights of the predominant metabolites from solid culture generally deceased with the ascending of the salinity and they were mostly small molecules with multiple hydroxyl and amino groups,which are speculated to involve the adjustment of osmotic pressure.[Conclusion]Salinity exerts a significant influence on the bioactivity and secondary metabolites of this strain.
different salinitiesmarine fungimolecular networksecondary metabolitesbiological activity
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