Physiological and Metabolic Mechanisms of Procambarus clarkii in Response to High Temperature Stress
[Objective]To understand the physiological and metabolic mechanisms of Procambarus clarkii in response to high temperature stress,and to provide references for improving P.clarkii culture management and variety breeding.[Method]The P.clarkii were exposed to 32℃and 37℃,and 26℃serving as the control condition.Gill samples were collected at 24 and 72 h post-exposure.The study employed a comprehensive analysis of histological observation,biochemical indicators(malondialdehyde,reactive oxygen species,total antioxidant capacity,pyruvate kinase,hexokinase,alkaline phosphatase,phosphoenolpyruvate carboxykinase,lysozyme,and acid phosphatase),and transcriptome to comprehensively explore the physiological and metabolic responses of P.clarkii to high temperature stress.[Result]High temperature damaged the gill tissues and the biochemical indicators overall decreased with the increasing of temperature.When compared to the control group,a total of 226 and 6 937 differentially expressed genes(DEGs)were identified at 24 and 72 h in 32℃group,and a total of 2 928 and 9 449 DEGs were identified at 24 and 72 h in 37℃group.GO and KEGG enrichment analyses indicate that DEGs were significantly enriched in O-glycan biosynthesis,extracellular matrix-receptor interaction,arachidonic acid metabolism,sphingolipid metabolism,as well as starch and sucrose metabolism processes.Additionally,the DEGs were found to cluster in modules pertinent to immune and energy metabolism based on weighted gene co-expression network analysis(WGCNA),hub genes like histones(H2A)and mitochondrial components(ND6)were identified.Ten DEGs were randomly selected to validate the RNA-seq results using reverse transcription-quantitative polymerase chain reaction(RT-qPCR),and the results and the expression profiles of these DEGs were of a well consistent with the high-throughput data,which confirmed the reliability of transcriptome results.[Conclusion]At temperatures of 32℃and 37℃,the gills of P.clarkii are damaged.The glucose utilization rate of P.clarkii is reduced in high temperature environments,causing cellular function to be maintained through the adjustments of heat shock proteins,antioxidant capacity,and metabolism.
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