Construction Strategies of Allosteric Transcription Factor Biosensors and Their Application Advances in Food Safety
With the rise of synthetic biology,transcription factor-based biosensors are gradually transitioning from in vivo to in vitro sensing.These sensors play a role in various detection fields,especially in the field of food safety,due to their high safety,strong stability,and fast response.Currently,most of the reviews on allosteric transcription factor(aTF)biosensors focus on construction of whole-cell biosensors in vivo.However,drawing on previous studies,this paper focuses on exploring the in vitro construction of aTF biosensors,such as using cell-free transcription-translation systems and compatible buffer systems as reaction vectors.In this paper,we provide a detailed review of the construction strategies of aTF-based in vitro biosensors and the progress of their application in food safety detection.Firstly,the construction of aTF biosensors is systematically described,including the molecular recognition mechanism of aTF,two signal amplification strategies of isothermal amplification and CRISPR-Cas,two signal output modes of optics and electrochemistry,as well as the use of two sensing systems,namely,the compatibility buffer and cell-free.Secondly,the progress of the application of aTF biosensors in the detection of food contaminants such as heavy metal ions,pesticide and veterinary drug residues,food additives,and foodborne pathogens is highlighted.Finally,the challenges faced by aTF biosensors are discussed in depth,and their future development trends are envisioned with a view to further expanding their potential applications in new fields.
万方数据
维普
