Cloning and Expression Analysis of CabZIP42 Gene in Pepper
[Objective]To explore the role of bZIP gene in response to abiotic stress would provide genetic resources for improving the stress resistance of peppers.[Method]A candidate gene CabZIP42 was obtained and cloned based on transcriptome sequencing in pepper 4 G7',the molecular characteristics of the encoded protein were analyzed by bioinformatics methods,and its expression patterns were analyzed by RT-qPCR in different tissues and under different stress treatments.[Result]The coding region of CabZIP42 was 1 233 bp in length,encoding 410 amino acids,with a predicted molecular weight of 44.85 kD and a theoretical isoelectric point of 9.47.The CabZIP42 protein contained two conserved domains,the basic amino acid region N-x9-R and the leucine zipper region x6-L-x6-L-x6-L.The secondary structure of the protein was dominated by random coils,and contained a small amount of α-helix.Phylogenetic analysis showed that CabZIP42 had the most recent protein evolutionary relationship with tomato and potato.Promoter prediction analysis showed that there were multiple hormonal and stress-related cis-acting elements on the CabZIP42 promoter.[Conclusion]The expression of CabZIP42 gene was the highest in pepper leaves,and it was involved in ABA signal transduction in response to drought,high temperature and high salt stress.
万方数据
维普
