Identification of Aux/IAA Gene Family in Corydalis hendersonii Hemsl.and Analysis on Their Expression Pattern under UVB Treatment
[Objective]To identify the Aux/IAA gene family in Corydalis hendersonii,and to provide a theoretical basis for the study of the function of C.hendersonii Aux/IAA genes and their resistances to adverse stresses.[Method]Based on the full-length transcriptome data of C.hendersonii,the members of the Aux/IAA(ChAux-IAA)gene family were identified and analyzed,and their proteins'physicochemical properties,phylogenetic tree,gene structure,protein tertiary structure,and protein interaction relationships were analyzed.In addition,C.Hendersonii was treated with UV-B at different times(0.5 h and 48 h)to detect the expression patterns of the ChAux/IAA gene family.[Result]The ChAux/IAA gene family of C.hendersonii comprises a total of 20 members,most of which are located in the cell nucleus,with a minority found in chloroplasts,mitochondria,and cell walls.The proteins encoded by the ChAux/IAA gene family are hydrophilic.The presence of light-responsive elements in the cis-acting elements of the ChAux/IAA gene family suggests that this gene family may be involved in plant growth and development and in resisting ultraviolet stress.Analysis via RT-qPCR revealed that under UVB treatment,the expressions of all 20 ChAux/IAA members increased compared to the control group,with ChIAA2,ChIAA3,ChIAA4,ChIAA10,ChIAA13 and ChIAA21 showing a more significant increase in expression.This indicates that the ChAux/IAA gene family plays an important role in the plant's resistance to ultraviolet stress.[Conclusion]A total of 20 ChAux/IAA gene family members are identified,and the ChAux/IAA gene family plays an important role in UV-B stress.
Corydalis hendersonii Hemsl.Aux/IAA gene familyUV-B stresstranscriptomebioinformaticsanalysis on gene expression
万方数据
维普
