Identification of the Promoter for the Biosynthesis Gene of Polyketide Meroterpenoids in Phomopsis tersa FS441
[Objective]Phomeroids are novel skeleton polyketide meroterpenoids isolated from the marine fungus Phomopsis tersa FS441,demonstrating promising anti-tumor activity.To facilitate the transcriptional regulation of the crucial genes for the biosynthesis of phomeroids,including the terpene cyclase gene ctg1509 and the P450 monooxygenase gene ctg1511,the functions for the promoters of these two genes were identified in this study.[Method]This study cloned the promoter p1509 and p1511 of ctg1509 and ctg1511 genes in marine fungus P.tersa FS441.The transcriptional activities of the p1509 and p1511 promoters were validated using luciferase reporter gene vectors,the plate experiment and the growth curve were employed to identify the function of the two promoters.Moreover,PlantCARE database was used to analyze the regulatory elements of the promoter.[Result]The promoter of p1509 has the strongest transcriptional activity,which is stronger than the positive promoter PgpdA.p1509 and p1511 promoters can initiate the transcription of ampicillin resistant gene AmpR in Escherichia coli.p1509 and p1511 promoters'regions not only contain core components such as CAAT box and TATA box,but also include cis-acting regulatory elements involved in low temperature response and light response.[Conclusion]This study confirmed the function of p1509 and p1511 promoters,and excavated a novel strong promoter,p1509,which had stronger transcriptional activity than the positive control and contained abundant regulatory elements.
promoters for biosynthetic genestranscriptional activityPhomopsis tersapolyketide meroterpenoidselement analysis
万方数据
维普
