Whole Genome Sequencing and Genome Evolution Analysis of Capsular Serotype A and D Pasteurella multocida of Bovine
[Objective]The capsular serotype of the Pasteurella multocida isolated from bovine in the Wulagai management area was identified.Whole genome sequencing and genome evolution analysis were performed for the strains with different serotypes.[Method]Taking the lung tissue samples from bovine for pathogen isolation and purification,the 16S rRNA identification,capsular serotype typing,drug sensitivity experiments,and mouse pathogenicity experiments were performed.Whole-genome sequencing and genomic evolution analysis were performed for the strains with different serotypes.[Result]Two strains of bovine Pasteurella multocida were successfully isolated,designated as Pm-YQ and Pm-SM.The Pm-YQ strain was capsular serotype A and the Pm-SM strain was capsular serotype D.Both strains presented relatively high virulence but low resistance to antibiotics.The whole genome lengths of the isolates were 2 274 102 bp and 2 244 957 bp,respectively,encoding 2 070 and 2 007 genes.The Pm-YQ strain belonged to sequence type ST 179,while Pm-SM strain belonged to ST 1.Through the construction of whole-genome phylogenetic tree,Pm-YQ strain was found to be closely related to three strains registered in GenBank from Germany,Denmark,and the United States,Pm-SM strain was found in the same evolutionary branch with a capsular serotype F of P.multocida strain isolated from Chongqing,China.[Conclusion]The isolation,identification and whole genome sequencing of two strains with different capsular serotypes of bovine P.multocida were completed.The Pm-YQ strain was closely related to the German isolate,and the Pm-SM strain was relatively far related to the currently registered bovine P.multocida strains.
万方数据
维普
