Roles of miR-3604 on the Receptivity,Proliferation,and Apoptosis of Bovine Endometrial Epithelial Cells
[Objective]To investigate the role of miR-3604 in the receptivity of bovine endometrial epithelial cells(bEECs).[Method]Progesterone(P4)and interferon tau(IFN-τ)were used to induce bEECs to construct a tolerant cell model.On this basis and after the overexpression and interference of bovine miR-3604 in the tolerant bEECs,the RT-qPCR,flow cytometry,EdU and CCK8 techniques were used to detect the receptivity of bovine miR-3604 in bEECs.Role in cell proliferation and apoptosis.[Result]The expressions of receptive marker gene HOXA10,IL-6 and VEGF significantly reduced in the miR-3604 overexpression group of receptive bEECs(P＜0.05).The expressions of apoptosis marker gene CASP3 and BAX were significantly up-regulated(P＜0.05).The apoptosis rate of acceptive bEECs significantly decreased(P＜0.01),while the cell proliferation rate significantly increased(P＜0.05).At the same time,the results of the miR-3604 interference experiment were contrary to the results of the overexpression experiment.[Conclusion]The expression of miR-3604 in receptive bEECs increased,which may inhibit the receptivity and apoptosis of bEECs,and promote the proliferation of bEECs.
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