Identification of β-amylase Gene Family in Melon and Their Response to Abiotic Stresses
[Objective]β-amylase(BAM)catalyzes the hydrolysis of starch and plays a crucial role in plant growth and stress response.Identifying the BAM gene family members from the whole melon genome may provide genetic resources for function study of BAM gene and resistance breeding in melon.[Method]This work analyzed the physicochemical properties,phylogenetic evolution,gene structure,conserved motifs,promoter cis-acting regulatory elements of the BAM family's protein.RT-qPCR was used to analyze their expression patterns under drought,ABA,low temperature,and salt stress.[Result]Nine CmBAMs were identified from the whole-genome of melon(Cucumis melo),and they were located on chromosome 1,4,5,6,7,and 11,with an average length of 543 amino acids.The average molecular weight was approximately 58.7 kD,and the isoelectric point ranged from 5.47 to 8.89.Phylogenetic analysis demonstrated that BAMs in melon were highly homologous with cucumbers(Cucumis sativus)with over 94.5%homology and one-to-one correspondence.CmBAMs promoter in melon mainly harbored hormone-responsive elements,photo-responsive elements,abiotic stress elements,and protein binding elements.Expression pattern analysis of CmBAMs under drought,ABA,low temperature,and salt stresses revealed that CmBAMs responded to these four abiotic stresses at varied level.Notably,CmBAM1,CmBAM3,and CmBAM9 were dramatically induced by drought,while CmBAM3,CmBAM5,and CmBAM9 showed strong induction by ABA.CmBAM1,CmBAM5,and CmBAM9 were obviously induced by cold stress whereas CmBAM3 showed the highest induction under salt stress.[Conclusion]Nine CmBAMs are identified from the whole genome of melon,and CmBAM1,CmBAM3,CmBAM5,and CmBAM9 were the most sensitive to the four abiotic stresses,suggesting that they might play a key role in abiotic stress and thus can be selected as candidate genes for further research.
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