首页|Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells

Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells

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Human umbilical cord mesenchymal stem cells (HUC-MSCs) are pluripotent and functional in many biological processes,by which releasing secretary factors to promote the self-repairing of damaged tis-sue or developing into functional cell at local organ.However,there is a high risk that oxidative stress would reduce the pluripotency and factor-secretion during the preparation and transplantation.There-fore,reducing oxidative stress is expected to improve the efficacy of HUC-MSCs therapy.Zinc (Zn) is an essential trace element which involves in the resistance of oxidative stress.To investigate Zn-regulated signaling pathways,we have profiled the gene expression at transcriptome level in primary HUC-MSCs treated with zinc sulfate,followed with GO and KEGG gene enrichment analysis.Zn treatment im-proved signal pathways for mineral absorption,cell growth,and cell death.Zn deficiency was mim-icked by TPEN administration,which suppressed cell proliferation and reduced the expression of HUC-MSCs surface stem cell markers CD73,CD90 and CD105 by flow cytometry.Nuclear factor erythrocyte 2 related factor 2 (Nrf2) plays an important role in antioxidant biological processes.In vitro treatment of Zn significantly increased Nrf2 and Sirt3 expression at gene level and protein level respectively.Zn supplementation inhibited TPEN-induced failure of cell survival and reversed the reduction of Nrf2 and Sirt3 expression,which further reduced the production of ROS.Zn successfully presented its anti-oxidation effect by activating Nrf2/Sirt3 signaling pathway in HUC-MSCs.Zn supplementation may im-prove the efficacy of HUC-MSCs therapy with reduced oxidative stress.

ZincOxidative stressNuclear factor erythroid-2-related factor 2Sirtuin 3Human umbilical cord mes-enchymal stem cells

Xiaodan Lu、Yifan Lin、Xiuying Lin、Qiang Zhang、Zihang Wang、Xuguang Mi、Ruobing Wang、Xiaofang Zhang、Xu Luan、Yan Liu、Bing Li、Yan Tan、Yanqiu Fang

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Diagnostic Medical Center,Jilin Province People's Hospital,Changchun 130021,China

School of Medicine,Changchun University of Chinese Medicine,Changchun 130021,China

Department of Clinical Laboratory,the Second Hospital of Jilin University,Changchun 130041,China

General Surgery,the First Hospital of Jilin University,Changchun 130021,China

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This study was funded by Jilin Province Science and Technology FundThis study was funded by Jilin Province Science and Technology FundThis study was funded by Jilin Province Science and Technology FundThis study was funded by Jilin Province Science and Technology FundNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaJilin Talents Developing FundHealth Commission of Jilin ProvinceHealth Commission of Jilin ProvinceHealth Commission of Jilin ProvinceHealth Commission of Jilin Province

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2021

生物物理学报
中国生物物理学会 中国科学院生物物理研究所

生物物理学报

影响因子：0.355
ISSN：1000-6737
年,卷(期)：2021.7(2)
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