Screening for metastasis-related genes in mouse melanoma cells through sequential tail vein injection

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外文摘要：Tumor metastasis,responsible for approximately 90％of cancer-associated mortality,remains poorly understood.Here in this study,we employed a melanoma lung metastasis model to screen for metastasis-related genes.By sequential tail vein injection of mouse melanoma B16F10 cells and the subsequently derived cells from lung metastasis into BALB/c mice,we successfully obtained highly metastatic B16F15 cells after five rounds of in vivo screening.RNA-sequencing analysis of B16F15 and B16F10 cells revealed a number of differentially expressed genes,some of these genes have previously been associated with tumor metastasis while others are novel discoveries.The identification of these metastasis-related genes not only improves our understanding of the metastasis mechanisms,but also provides potential diagnostic biomarkers and therapeutic targets for metastatic melanoma.

外文关键词：

MelanomaLung metastasis modelGene screeningTail vein injection

作者：

Qinggang Hao、Rui Dong、Weiyu Bai、Dong Chang、Xinyi Yao、Yingru Zhang、Huangying Xu、Huiyan Li、Xiang Kui、Feng Wang、Yan Wang、Chengqin Wang、Yujie Lei、Yan Chen、Junling Shen、Lei Sang、Yan Bai、Jianwei Sun

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作者单位：

Center for Life Sciences,Yunnan Key Laboratory of Cell Metabolism and Diseases,State Key Laboratory for Con-servation and Utilization of Bio-Resources in Yunnan,School of Life Sciences,Yunnan University,Kunming 650500,China

Key Laboratory of Tumor Immunological Prevention and Treatment of Yunnan Province,Yan'an Hospital Affiliat-ed to Kunming Medical University,Kunming 650051,China

Center for Life Sciences,Yunnan Key Laboratory of Cell Metabolism and Diseases,State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,School of Life Sciences,Yunnan University,Kunming 650500,China

Shandong Provincial Key Laboratory of Animal Resistance Biology,Collaborative Innovation Center of Cell Biolo-gy in Universities of Shandong,Center for Cell Structure and Function,Institute of Biomedical Science,College of Life Sciences,Shandong Normal University,Jinan 250014,China

Department of Pathology,The Second Affiliated Hospital of Kunming Medical University,Kunming 650101,China

State Key Laboratory of Bioactive Molecules and Druggability Assessment,Institute of Genomic Medicine,College of Pharmacy,Jinan University,Guangzhou 510632,China

Department of Pathology,School of Basic Medicine,Qingdao University,Qingdao 266000,China

Department of Thoracic Surgery,Yunnan Cancer Hospital & The Third Affiliated Hospital of Kunming Medical University & Yunnan Cancer Center,Kunming 650106,China

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基金：

国家自然科学基金国家自然科学基金Yunnan Applied and Basic Research ProgramYunnan Applied and Basic Research ProgramTaishan Scholar Young Expert Program of Shandong ProvinceMajor Science and Technique Programs in Yunnan ProvinceKey Laboratory of Tumor Immunological Prevention and Treatment in Yunnan ProvinceYan'an Hospital Affliated to Kunming Medical University from Yunnan UniversityYan'an Hospital Affliated to Kunming Medical University from Yunnan UniversityYan'an Hospital Affliated to Kunming Medical University from Yunnan UniversityYan'an Hospital Affliated to Kunming Medical University from Yunnan UniversityYan'an Hospital Affliated to Kunming Medical University from Yunnan UniversityExternal Cooperation Research Project from the Second Affiliated Hospital of Kunming Medical University

项目编号：

8227346032260167202101AV070002202301AS070117tsqn202306155202202AA310207KLTIPT-2023-02KC-23233927KC-23234451ZC-23236399ZC-23236296TM-232369242022dwhz13

出版年：

2024

DOI：

10.52601/bpr.2023.230043

生物物理学报告(英文)

ISSN：

年,卷(期)：2024.10(1)