首页|An optimized method of culturing neurons based on polyacrylamide gel

An optimized method of culturing neurons based on polyacrylamide gel

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Substrate stiffness is a microenvironment with a certain stiffness constructed by the extracellular ma-trix and adjacent cells,which plays an important role in the growth and development of cells and tis-sue formation.Studies have indicated that the stiffness of the brain is about 0.1-1 kPa.The physiologi-cal and pathological processes of the nervous system are mediated by the substrate stiffness that the neurons suffer.However,how substrate stiffness regulates these processes remains to be studied.Cul-turing neurons on substrates with different stiffness in vitro is one of the best methods to study the role of stiffness in regulating neuronal development and activity.In this study,by changing the preparation time and the activation time of polyacrylamide gel,we provide an improved method that achieves a low toxic substrate environment for better primary neuron adhesion and development.Hope that this method is convenient for those studying the role of substrate stiffness in neurons.

Polyacrylamide gelNeuron cultureCell adhesionNeural cell development

Yongjing Qiao、Jihong Gong、Ziqi Jin、Yiting Tu、Xiaofei Yang

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Key Laboratory of Cognitive Science,Hubei Key Laboratory of Medical Information Analysis and Tumor Diagno-sis & Treatment,Laboratory of Membrane Ion Channels and Medicine,College of Biomedical Engineering,South-Central Minzu University,Wuhan 430074,China

国家自然科学基金国家自然科学基金National Science Foundation of HubeiKnowledge Innovation Program of Wuhan-ShuguangFundamental Research Funds for the Central Universities of South-Central Minzu University

32170699322005602022CFB906SZY23007CZQ22005

2024

10.52601/bpr.2023.230033

生物物理学报告(英文)

生物物理学报告(英文)

ISSN：
年,卷(期)：2024.10(1)