首页|Enrichment of ER tubule-derived microsomes by differential centrifugation and immunoprecipitation
Enrichment of ER tubule-derived microsomes by differential centrifugation and immunoprecipitation
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The endoplasmic reticulum(ER)is an essential component of the endomembrane system in eukary-otes and plays a crucial role in protein and lipid synthesis,as well as the maintenance of calcium homeo-stasis.Morphologically,the ER is composed primarily of sheets and tubules.The tubular ER is com-posed of a network of tubular membrane structures,each with diameters ranging from 30 to 50 nanometers.In recent years,there has been in-depth research on the molecular mechanisms of mem-brane shaping and membrane fusion of the tubular ER.However,there is still limited understanding of the specific physiological functions of the tubular ER.Here,we report a protocol that combines differ-ential centrifugation and immunoprecipitation to specifically enrich microsomes originating from the tubular ER in yeast.The ER tubule-derived microsomes can be further used for proteomic and lipido-mic studies or other biochemical analyses.
National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Bio-physics,Chinese Academy of Sciences,Beijing 100101,China
University of Chinese Academy of Sciences,Beijing 101408,China
国家重点研发计划Project for Young Scientists in Basic Research of the Chinese Academy of Sciences
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