首页|Detection of ribophagy in yeast and mammals

Detection of ribophagy in yeast and mammals

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Ribophagy,the cellular process wherein ribosomes are selectively self-digested through autophagy,plays a pivotal role in maintaining ribosome turnover.Understanding the molecular regulatory mecha-nisms governing ribophagy is pivotal to uncover its significance.Consequently,the establishment of methods for detecting ribophagy becomes important.In this protocol,we have optimized,enriched,and advanced existing ribophagy detection techniques,including immunoblotting,fluorescence mi-croscopy,and transmission electron microscopy(TEM),to precisely monitor and quantify ribophagic events.Particularly noteworthy is the introduction of TEM technology for yeast ribophagy detection.In summary,the delineated methods are applicable for detecting ribophagy in both yeast and mammals,laying a solid foundation for further exploring the physiological importance of ribophagy and its poten-tial implications in diverse cellular environments.

RibophagyRibosome turnoverImmunoblottingFluorescence microscopyTransmission electron mi-croscopyYeast and mammals

Miao Ye、Yuting Chen、Zhaojie Liu、Yigang Wang、Cong Yi

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Xinyuan Institute of Medicine and Biotechnology,School of Life Sciences and Medicine,Zhejiang Sci-Tech Univer-sity,Hangzhou 310018,China

Department of Biochemistry,and Department of Hepatobiliary and Pancreatic Surgery of the First Affiliated Hos-pital,Zhejiang University School of Medicine,Hangzhou 310058,China

Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation,Affili-ated Cancer Hospital & Institute of Guangzhou Medical University,School of Basic Medical Sciences,Guangzhou Medical University,Guangzhou 510182,China

2024

10.52601/bpr.2024.240002

生物物理学报告(英文)

生物物理学报告(英文)

ISSN：
年,卷(期)：2024.10(2)
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