首页|Co-immunoprecipitation for identifying protein-protein interaction on lipid droplets
Co-immunoprecipitation for identifying protein-protein interaction on lipid droplets
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The lipid droplet(LD)is a conserved organelle that exists in almost all organisms,ranging from bacte-ria to mammals.Dysfunctions in LDs are linked to a range of human metabolic syndromes.The forma-tion of protein complexes on LDs is crucial for maintaining their function.Investigating how proteins interact on LDs is essential for understanding the role of LDs.We have developed an effective method to uncover protein-protein interactions and protein complexes specifically on LDs.In this method,we conduct co-immunoprecipitation(co-IP)experiments using LD proteins extracted directly from isolat-ed LDs,rather than utilizing proteins from cell lysates.To elaborate,we begin by purifying LDs with high-quality and extracting LD-associated proteins.Subsequently,the co-IP experiment is performed on these LD-associated proteins directly,which would enhance the co-IP experiment specificity of LD-associated proteins.This method enables researchers to directly unveil protein complexes on LDs and gain deeper insights into the functional roles of proteins associated with LDs.
Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China
University of Chinese Academy of Sciences,Beijing 100049,China
Institute of Infectious Diseases,Beijing Key Laboratory of Emerging Infectious Diseases,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China
Beijing Institute of Infectious Diseases,Beijing 100015,China
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