首页|Morphological determination of localization and function of Golgi proteins
Morphological determination of localization and function of Golgi proteins
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In animal cells,the Golgi apparatus serves as the central hub of the endomembrane secretory pathway.It is responsible for the processing,modification,and sorting of proteins and lipids.The unique stack-ing and ribbon-like architecture of the Golgi apparatus forms the foundation for its precise functionali-ty.Under cellular stress or pathological conditions,the structure of the Golgi and its important glyco-sylation modification function may change.It is crucial to employ suitable methodologies to study the structure and function of the Golgi apparatus,particularly when assessing the involvement of a target protein in Golgi regulation.This article provides a comprehensive overview of the diverse microscopy techniques used to determine the specific location of the target protein within the Golgi apparatus.Ad-ditionally,it outlines methods for assessing changes in the Golgi structure and its glycosylation modifi-cation function following the knockout of the target gene.
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NSTL
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