Streamlined process for effective and strand-selective mitochondrial base editing using mitoBEs

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外文摘要：Mitochondrial base editing tools hold great promise for the investigation and treatment of mitochon-drial diseases.Mitochondrial DNA base editors(mitoBEs)integrate a programmable transcription-activator-like effector(TALE)protein with single-stranded DNA deaminase(TadA8e-V106W,APOBEC1,etc.)and nickase(MutH,Nt.BspD6I(C),etc.)to achieve heightened precision and efficiency in mitochondrial base editing.This innovative mitochondrial base editing tool exhibits a number of ad-vantages,including strand-selectivity for editing,high efficiency,and the capacity to perform diverse types of base editing on the mitochondrial genome by employing various deaminases.In this context,we provide a detailed experimental protocol for mitoBEs to assist others in achieving proficient mito-chondrial base editing.

外文关键词：

Mitochondrial DNA base editors(mitoBEs)Transcription-activator-like effector(TALE)DeaminaseNickase

作者：

Xiaoxue Zhang、Zongyi Yi、Wei Tang、Wensheng Wei

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作者单位：

Changping Laboratory,Beijing 102206,China

Biomedical Pioneering Innovation Center,Peking-Tsinghua Center for Life Sciences,Peking University Genome Editing Research Center,State Key Laboratory of Protein and Plant Gene Research,School of Life Sciences,Peking University,Beijing 100871,China

Academy for Advanced Interdisciplinary Studies,Peking University,Beijing 100871,China

出版年：

2024

DOI：

10.52601/bpr.2024.240010

生物物理学报告(英文)

ISSN：

年,卷(期)：2024.10(4)